{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T08:18:01Z","timestamp":1759565881659},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"9","license":[{"start":{"date-parts":[[1971,9,1]],"date-time":"1971-09-01T00:00:00Z","timestamp":52531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Biochem."],"published-print":{"date-parts":[[1971,9,1]]},"abstract":"<jats:p> Human plasminogen was prepared from Cohn fraction III by affinity chromatography with 35-fold purification and 70% recovery. The solubility of this plasminogen at pH 7.4 depends on the pH of the solutions in which it was kept during the purification procedure. Thus, a native and an altered form can be obtained by using 0.1\u2002M lysine at pH 7.4 and 0.1\u2002M acetic acid at pH 3.0, respectively, as eluting agents. The altered plasminogen has high activity when suspended in acidic solution. It shows little activity when suspended in pH 7.4 buffer; its activity is partially restored with addition of 0.1\u2002M lysine. The specific activity of these plasminogens is in the same range as that of plasminogen prepared directly from human plasma by affinity chromatography. <\/jats:p>","DOI":"10.1139\/o71-154","type":"journal-article","created":{"date-parts":[[2009,12,17]],"date-time":"2009-12-17T22:13:43Z","timestamp":1261088023000},"page":"1055-1061","source":"Crossref","is-referenced-by-count":26,"title":["Studies on Plasminogen. IX. Purification of Human Plasminogen from Cohn Fraction III by Affinity Chromatography"],"prefix":"10.1139","volume":"49","author":[{"given":"T. H.","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edwin T.","family":"Mertz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/o71-154","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T22:50:09Z","timestamp":1578264609000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/o71-154"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,9,1]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1971,9,1]]}},"alternative-id":["10.1139\/o71-154"],"URL":"https:\/\/doi.org\/10.1139\/o71-154","relation":{},"ISSN":["0008-4018"],"issn-type":[{"value":"0008-4018","type":"print"}],"subject":[],"published":{"date-parts":[[1971,9,1]]}}}