{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T22:26:43Z","timestamp":1791844003627,"version":"4.3.4"},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"9","license":[{"start":{"date-parts":[[1973,9,1]],"date-time":"1973-09-01T00:00:00Z","timestamp":115689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Biochem."],"published-print":{"date-parts":[[1973,9,1]]},"abstract":"<jats:p> Unlike other phage systems, the development of PBS 1 was found to be insensitive to rifamycin SV. The incorporation of <jats:sup>3<\/jats:sup>H-uridine into trichloroacetic acid precipitable and alkali-labile material (RNA), in PBS-1-infected cells, was greatly reduced by rifamycin. Observations that RNA synthesized in the presence of rifamycin was hybridizable exclusively with the phage DNA and that actinomycin D inhibited the phage growth indicated that the synthesis of a new species of RNA was required for the development of PBS 1. The host DNA synthesis was reduced to a very low level 5\u2002min after infection. The phage DNA synthesis was also apparently reduced markedly by rifamycin when determined with <jats:sup>3<\/jats:sup>H-uridine as labelling material. On the other hand, rifamycin did not affect the incorporation of <jats:sup>3<\/jats:sup>H-deoxycytidine into the phage DNA, suggesting that phage DNA synthesis was in fact insensitive to rifamycin. It is not clear how rifamycin inhibits the incorporation of <jats:sup>3<\/jats:sup>H-uridine into nucleic acids in PBS-1-infected cells. <\/jats:p>","DOI":"10.1139\/o73-161","type":"journal-article","created":{"date-parts":[[2009,12,18]],"date-time":"2009-12-18T16:13:14Z","timestamp":1261152794000},"page":"1219-1224","source":"Crossref","is-referenced-by-count":16,"title":["The Synthesis of Nucleic Acids in <i>Bacillus subtilis<\/i> Infected with Phage PBS 1"],"prefix":"10.1139","volume":"51","author":[{"given":"B. K.","family":"Rima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","container-title":["Canadian Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/o73-161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T17:49:12Z","timestamp":1578246552000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/o73-161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,9,1]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1973,9,1]]}},"alternative-id":["10.1139\/o73-161"],"URL":"https:\/\/doi.org\/10.1139\/o73-161","relation":{},"ISSN":["0008-4018"],"issn-type":[{"value":"0008-4018","type":"print"}],"subject":[],"published":{"date-parts":[[1973,9,1]]}}}