{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T15:41:34Z","timestamp":1649000494453},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"9","license":[{"start":{"date-parts":[[1976,9,1]],"date-time":"1976-09-01T00:00:00Z","timestamp":210384000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Biochem."],"published-print":{"date-parts":[[1976,9,1]]},"abstract":"<jats:p> Homogenates of the free-living amoeba Acanthamoeba castellanii incorporate phosphate from [\u03b3-<jats:sup>32<\/jats:sup>P]ATP into a lipid which co-chromatographs with diphosphoinositide on one- and two-dimensional chromatography. Incorporation into lipids similar in mobility to triphosphoinositide is not detected. The product co-chromatographs with diphosphoinositide whether exogenous phosphatidylinositol or total amoeba lipid is the substrate. The inositide kinase is almost entirely located in the supernatant fraction after centrifugation at 100\u2002000\u2002g. Incorporation of phosphate from [\u03b3-<jats:sup>32<\/jats:sup>P]ATP is linear for at least 15\u2002min in the presence of 0.5\u2002mM phosphatidylinositol. The enzyme requires Mg<jats:sup>2+<\/jats:sup> or Mn<jats:sup>2+<\/jats:sup> as well as ATP and it is not affected by low concentrations of Ca<jats:sup>2+<\/jats:sup>. The apparent K<jats:sub>m<\/jats:sub> for phosphatidylinositol is 2\u2002mM. Both ADP and cAMP inhibit the reaction. <\/jats:p>","DOI":"10.1139\/o76-110","type":"journal-article","created":{"date-parts":[[2009,12,18]],"date-time":"2009-12-18T16:17:56Z","timestamp":1261153076000},"page":"772-777","source":"Crossref","is-referenced-by-count":3,"title":["Synthesis of diphosphoinositide by a supernatant fraction from <i>Acanthamoeba castellanii<\/i>"],"prefix":"10.1139","volume":"54","author":[{"given":"J. Thomas","family":"Buckley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/o76-110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T17:52:23Z","timestamp":1578246743000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/o76-110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,9,1]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1976,9,1]]}},"alternative-id":["10.1139\/o76-110"],"URL":"https:\/\/doi.org\/10.1139\/o76-110","relation":{},"ISSN":["0008-4018"],"issn-type":[{"value":"0008-4018","type":"print"}],"subject":[],"published":{"date-parts":[[1976,9,1]]}}}