{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T14:30:20Z","timestamp":1756996220684},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"5","license":[{"start":{"date-parts":[[1978,5,1]],"date-time":"1978-05-01T00:00:00Z","timestamp":262828800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Biochem."],"published-print":{"date-parts":[[1978,5,1]]},"abstract":"<jats:p> The effects of L-ascorbic acid deficiency on guinea pig hepatic and brain lysosomal hydrolases were examined. In general, hepatic \u03b2-N-acetylhexosaminidase, \u03b2-D-glucoronidase, \u03b1-D-galactosidase, \u03b1-D-mannosidase, and acid phosphatase were elevated in scorbutic animals. This appears to be independent of the starved state. Brain \u03b2-D-glucoronidase and acid phosphatase followed a similar pattern to that observed with the liver enzymes, but brain \u03b2-N-acetylhexosaminidase was not affected by L-ascorbic acid deficiency. Supplementing the in vitro assay system with L-ascorbic acid decreased the activity of hepatic \u03b2-N-acetylhexosaminidase somewhat but had no effect on the brain enzyme. Serum total \u03b2-N-acetylhexosaminidase was unaffected by dietary treatments although the activity of \u03b2-N- acetylhexosaminidase A tended to increase in the scorbutic animals. Subcellular fractions were obtained from the three groups of animals and the recoveries of protein, \u03b2-N-acetylhexosaminidase, and glucose-6-phosphatase estimated. <\/jats:p>","DOI":"10.1139\/o78-056","type":"journal-article","created":{"date-parts":[[2009,12,21]],"date-time":"2009-12-21T09:51:49Z","timestamp":1261389109000},"page":"352-356","source":"Crossref","is-referenced-by-count":13,"title":["<scp>L<\/scp>-Ascorbic acid and lysosomal acid hydrolase activities of guinea pig liver and brain"],"prefix":"10.1139","volume":"56","author":[{"given":"Susan K.","family":"Hoehn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian N.","family":"Kanfer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/o78-056","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T17:52:46Z","timestamp":1578246766000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/o78-056"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1978,5,1]]}},"alternative-id":["10.1139\/o78-056"],"URL":"https:\/\/doi.org\/10.1139\/o78-056","relation":{},"ISSN":["0008-4018"],"issn-type":[{"value":"0008-4018","type":"print"}],"subject":[],"published":{"date-parts":[[1978,5,1]]}}}