{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T04:11:47Z","timestamp":1771647107363,"version":"3.50.1"},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"4","license":[{"start":{"date-parts":[[1979,4,1]],"date-time":"1979-04-01T00:00:00Z","timestamp":291772800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Biochem."],"published-print":{"date-parts":[[1979,4,1]]},"abstract":"<jats:p> The metabolism of glutamine by a suspension of rat kidney tubules was studied in vitro. The influence of duration of incubation, glutamine concentration, and metabolic state of the donor animals was investigated. The relative importance of glucose synthesis, amino acid production, and oxidation to CO<jats:sub>2<\/jats:sub> was estimated by drawing a complete balance of the nitrogens and the carbon chains of the extracted glutamine. It was found that the initial (first 15\u2002min) rate of glutamine utilization was significantly greater than the subsequent rate due to an initial, but transient, extracellular accumulation of glutamate. This phenomenon was suppressed when a small amount of glutamate was added to the incubation medium. Glucose production constitutes the major fate for glutamine metabolism. No net oxidation of glutamine could be detected with 1\u2002mM glutamine during the first 30\u2002min. However, glutamine oxidation becomes significant after prolonged incubation (16% at 120\u2002min). The metabolic fate of glutamine differs when 5 or 10\u2002mM are presented to the tubules, glutamate production and oxidation to CO<jats:sub>2<\/jats:sub> becoming more important. Metabolic acidosis or a 48-h fast increases glutamine extraction and enhances its utilization towards glucose synthesis while they depress glutamate accumulation and oxidation to CO<jats:sub>2<\/jats:sub>. Metabolic alkalosis has the opposite effect. It is concluded that the metabolism of glutamine in vitro is dependent on the conditions of the study. Furthermore, total oxidation to CO<jats:sub>2<\/jats:sub> is not a major fate for glutamine metabolism at physiological concentration and is not enhanced by acidosis in the rat kidney in vitro. <\/jats:p>","DOI":"10.1139\/o79-044","type":"journal-article","created":{"date-parts":[[2009,12,21]],"date-time":"2009-12-21T14:54:28Z","timestamp":1261407268000},"page":"346-356","source":"Crossref","is-referenced-by-count":14,"title":["Characteristics of glutamine metabolism by rat kidney tubules: a carbon and nitrogen balance"],"prefix":"10.1139","volume":"57","author":[{"given":"Patrick","family":"Vinay","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy","family":"Lemieux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9","family":"Gougoux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/o79-044","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T22:51:24Z","timestamp":1578264684000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/o79-044"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1979,4,1]]}},"alternative-id":["10.1139\/o79-044"],"URL":"https:\/\/doi.org\/10.1139\/o79-044","relation":{},"ISSN":["0008-4018"],"issn-type":[{"value":"0008-4018","type":"print"}],"subject":[],"published":{"date-parts":[[1979,4,1]]}}}