{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T18:47:11Z","timestamp":1648579631177},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"9","license":[{"start":{"date-parts":[[1979,9,1]],"date-time":"1979-09-01T00:00:00Z","timestamp":304992000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Biochem."],"published-print":{"date-parts":[[1979,9,1]]},"abstract":"<jats:p> The effects of alcohols on human glycophorin were monitored by circular dichroism, solvent perturbation of absorption spectra, fluorescence of 8-anilino-1-naphthalene sulfonate, and sedimentation equilibrium in the ultracentrifuge. Both ethanol and 2-chloroethanol gradually increase the \u03b1 helix in glycophorin and its sialic acid free counterpart. The same alcohols do not cause a cooperative transition in the structure of the polypeptide chain of glycophorin. Other alcohols also increase the \u03b1-helix content of glycophorin. Binding of ANS to glycophorin is abolished at relatively low alcohol concentrations. Ethanol at 60% (v\/v) reduces the molecular weight ratio of glycophorin and at the same time increases the exposure of tyrosine residues to solvent. These observations indicate a complex mechanism of interaction of weakly protic solvents with this stable membrane protein. <\/jats:p>","DOI":"10.1139\/o79-148","type":"journal-article","created":{"date-parts":[[2009,12,21]],"date-time":"2009-12-21T09:54:50Z","timestamp":1261389290000},"page":"1153-1158","source":"Crossref","is-referenced-by-count":1,"title":["The effects of alcohols on the structure of human glycophorin"],"prefix":"10.1139","volume":"57","author":[{"given":"David M.","family":"Byers","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob A.","family":"Verpoorte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/o79-148","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T17:51:32Z","timestamp":1578246692000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/o79-148"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,9,1]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1979,9,1]]}},"alternative-id":["10.1139\/o79-148"],"URL":"https:\/\/doi.org\/10.1139\/o79-148","relation":{},"ISSN":["0008-4018"],"issn-type":[{"value":"0008-4018","type":"print"}],"subject":[],"published":{"date-parts":[[1979,9,1]]}}}