{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T11:19:01Z","timestamp":1758280741113},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"10","license":[{"start":{"date-parts":[[1980,10,1]],"date-time":"1980-10-01T00:00:00Z","timestamp":339206400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Biochem."],"published-print":{"date-parts":[[1980,10,1]]},"abstract":"<jats:p> Cells of the marine bacterium Alteromonas haloplanktis 214 ATCC 19855 (previously referred to as marine pseudomonad B-16) were depleted of K<jats:sup>+<\/jats:sup> by washing with 0.1\u2002M MgSO<jats:sub>4<\/jats:sub>. Washing with 0.05\u2002M MgSO<jats:sub>4<\/jats:sub> lowered the V<jats:sub>max<\/jats:sub> for K<jats:sup>+<\/jats:sup> transport compared with washing with 0.1\u2002M but did not change the K<jats:sub>m<\/jats:sub>, while washing with lower concentrations of MgSO<jats:sub>4<\/jats:sub> caused loss of ultraviolet-absorbing material from the cells. K<jats:sup>+<\/jats:sup> uptake was a strictly aerobic process and was accompanied by proton release. When an anaerobic suspension of cells was added to incubation mixtures containing increasing amounts of O<jats:sub>2<\/jats:sub>, intracellular ATP concentrations increased as the O<jats:sub>2<\/jats:sub> concentration increased and reached near maximum values before K<jats:sup>+<\/jats:sup> transport began. The O<jats:sub>2<\/jats:sub> concentration initiating K<jats:sup>+<\/jats:sup> transport caused transport to proceed at its maximum rate. For these experiments A. haloplanktis was depleted of ATP by incubating under anaerobic conditions. Incubating with either N\u2032,N\u2032-dicyclohexyl carbodiimide (DCCD) or arsenate failed to deplete intact cells of ATP or prevent K<jats:sup>+<\/jats:sup> transport. The inhibitory activity of DCCD for ATPase in membrane preparations was higher at 5\u2002mM than at other MgSO<jats:sub>4<\/jats:sub> concentrations and increased with time. Cyanide and the uncoupling agents tetrachlorosalicylanide (TCS) and carbonylcyanide p-trifluoromethoxyphenylhydrazone (FCCP) prevented K<jats:sup>+<\/jats:sup> uptake while TCS and FCCP though not cyanide caused K<jats:sup>+<\/jats:sup> to be released from K<jats:sup>+<\/jats:sup>-containing cells. It is concluded that the driving force for K<jats:sup>+<\/jats:sup> transport in these cells is likely to be the membrane potential and that K<jats:sup>+<\/jats:sup> transport may be gated. <\/jats:p>","DOI":"10.1139\/o80-161","type":"journal-article","created":{"date-parts":[[2009,12,21]],"date-time":"2009-12-21T15:22:25Z","timestamp":1261408945000},"page":"1206-1214","source":"Crossref","is-referenced-by-count":6,"title":["Energy coupling to K<sup>+<\/sup> transport in a marine bacterium"],"prefix":"10.1139","volume":"58","author":[{"given":"Edward G.","family":"Sedgwick","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"MacLeod","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/o80-161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T22:52:10Z","timestamp":1578264730000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/o80-161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,10,1]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1980,10,1]]}},"alternative-id":["10.1139\/o80-161"],"URL":"https:\/\/doi.org\/10.1139\/o80-161","relation":{},"ISSN":["0008-4018"],"issn-type":[{"value":"0008-4018","type":"print"}],"subject":[],"published":{"date-parts":[[1980,10,1]]}}}