{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,13]],"date-time":"2024-07-13T13:14:55Z","timestamp":1720876495444},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"11","license":[{"start":{"date-parts":[[1984,11,1]],"date-time":"1984-11-01T00:00:00Z","timestamp":468115200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Biochem. Cell Biol."],"published-print":{"date-parts":[[1984,11,1]]},"abstract":"<jats:p> Human serum contains two classes of somatomedins (Sin) with similar three-dimensional structures and mass (i.e., ~7500 kdaltons (kd)), but that can be distinguished from each other by their isoelectric point (pI) values. Those with alkaline pI's include the presumably identical molecules of insulin-like growth factor I (IGF-I), SmA, SmC, and basic Sm. In humans, the neutral\u2013acidic class of Sm is represented by IGF-II. The sera of rats and other animals contain similar classes of Sm. In normal children and adults, plasma levels of both IGF-I and -II are under growth hormone control, although only elevated concentrations of the former can be positively correlated with adolescent skeletal growth. Elevated blood levels of IGF-II or an embryonic form of it, however, are correlated with fetal development of rats, sheep, and possibly in humans. As evidenced from competitive radioreceptor binding studies and affinity-labelling techniques, receptors that bind insulin and IGF-I are immunologically, structurally, and functionally related: (a) monoclonal and polyclonal antibodies to insulin receptors recognize determinants on receptors that bind IGF-I; (b) both receptors have 140-, 95-, and possibly 45-kd subunits that are interchain disulfide-bonded to form glycoprotein complexes of relative masses (M<jats:sub>r<\/jats:sub>) 300\u2002000 \u2013 400\u2002000; and (c) occupancy of the 140-kd subunits by hormone or growth factor stimulates the phosphorylation of tyrosine residues on the 95-kd subunit by receptor-associated protein kinases. Receptors that bind IGF-II are very different from insulin or IGF-I receptors: (a) there is no evidence of immunological cross-reactivity to the insulin receptors or of intrinsic protein kinase activity and (b) estimates of the molecular weight of the detergent-solubilized IGF-II receptor from its hydrodynamic properties or from its electrophoretic mobility during quantitative or sodium dodecyl sulfate\u2013polyacrylamide gel electrophoresis established it to be a single-chained glycoprotein of MT 220\u2002000 held together by intrachain disulfide bonds. <\/jats:p>","DOI":"10.1139\/o84-158","type":"journal-article","created":{"date-parts":[[2009,12,23]],"date-time":"2009-12-23T19:01:30Z","timestamp":1261594890000},"page":"1237-1245","source":"Crossref","is-referenced-by-count":27,"title":["Chemistry, structure, and function of insulin-like growth factors and their receptors: a review"],"prefix":"10.1139","volume":"62","author":[{"given":"James F.","family":"Perdue","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Biochemistry and Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/o84-158","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T20:39:13Z","timestamp":1578256753000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/o84-158"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,11,1]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1984,11,1]]}},"alternative-id":["10.1139\/o84-158"],"URL":"https:\/\/doi.org\/10.1139\/o84-158","relation":{},"ISSN":["0714-7511"],"issn-type":[{"value":"0714-7511","type":"print"}],"subject":[],"published":{"date-parts":[[1984,11,1]]}}}