{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T06:07:34Z","timestamp":1769580454134,"version":"3.49.0"},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[1988,3,1]],"date-time":"1988-03-01T00:00:00Z","timestamp":573177600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. Cell Biol."],"published-print":{"date-parts":[[1988,3,1]]},"abstract":"<jats:p> The effect of an analogue of cAMP on the uptake and metabolism of choline in the heart was studied in isolated cardiac cells. The cells were obtained from 7-day-old chick embryos and maintained in culture. The effects of cAMP were studied using the dibutyryl cAMP analogue and the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine. After a 2-h incubation with [<jats:sup>3<\/jats:sup>H]choline, about 85% of the label was recovered in phosphocholine, with most of the rest in phospholipid. During a subsequent chase incubation, [<jats:sup>3<\/jats:sup>H]phosphocholine was transferred to phosphatidylcholine with little accumulation in CDP-choline. This suggests the rate-limiting step for the conversion of phosphocholine to phosphatidylcholine in these cells is the synthesis of CDP-choline. cAMP decreased the incorporation of choline into phosphatidylcholine, but did not change the flux of metabolites through the step catalyzed by CTP:phosphocholine cytidylyltransferase. cAMP had little effect on choline uptake at low (1\u201325\u2002\u03bcM) extracellular choline concentrations, but significantly (p\u2002&lt;\u20020.05) decreased choline uptake at higher (37.5\u201350\u2002\u03bcM) extracellular choline concentrations. Thus, cardiac cells take up and metabolize choline to phosphocholine, with CTP:phosphocholine cytidylyltransferase being the rate-limiting step in phosphatidylcholine biosynthesis. cAMP decreases [<jats:sup>3<\/jats:sup>H]choline uptake and its subsequent incorporation into phosphocholine and phospholipid. However, the metabolism of choline within the cell is unaffected. <\/jats:p>","DOI":"10.1139\/o88-030","type":"journal-article","created":{"date-parts":[[2009,12,23]],"date-time":"2009-12-23T19:36:00Z","timestamp":1261596960000},"page":"231-237","source":"Crossref","is-referenced-by-count":8,"title":["Effect of cAMP on choline uptake and phosphatidylcholine biosynthesis in cultured chick heart cells"],"prefix":"10.1139","volume":"66","author":[{"given":"Paul S.","family":"Sunga","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. Haydn","family":"Pritchard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon W.","family":"Rabkin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Biochemistry and Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdnsciencepub.com\/doi\/pdf\/10.1139\/o88-030","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T18:53:19Z","timestamp":1751482399000},"score":1,"resource":{"primary":{"URL":"https:\/\/cdnsciencepub.com\/doi\/10.1139\/o88-030"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1988,3,1]]}},"alternative-id":["10.1139\/o88-030"],"URL":"https:\/\/doi.org\/10.1139\/o88-030","relation":{},"ISSN":["0829-8211","1208-6002"],"issn-type":[{"value":"0829-8211","type":"print"},{"value":"1208-6002","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,3,1]]}}}