{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T04:17:29Z","timestamp":1751516249831,"version":"3.41.0"},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[1988,3,1]],"date-time":"1988-03-01T00:00:00Z","timestamp":573177600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. Cell Biol."],"published-print":{"date-parts":[[1988,3,1]]},"abstract":"<jats:p> The specificity of glycogen synthase (casein) kinase-1 (CK-1) for different divalent metal ions was explored in this study. Of nine metal ions (Mg<jats:sup>2+<\/jats:sup>, Mn<jats:sup>2+<\/jats:sup>, Zn<jats:sup>2+<\/jats:sup>, Cu<jats:sup>2+<\/jats:sup>, Ca<jats:sup>2+<\/jats:sup>, Ba<jats:sup>2+<\/jats:sup>, Ni<jats:sup>2+<\/jats:sup>, Co<jats:sup>2+<\/jats:sup>, Fe<jats:sup>2+<\/jats:sup>) tested, only Mg<jats:sup>2+<\/jats:sup> supported significant kinase activity. Several of the other metals, however, inhibited the Mg<jats:sup>2+<\/jats:sup>-stimulated kinase activity. Half-maximal inhibitions by Mn<jats:sup>2+<\/jats:sup>, Zn<jats:sup>2+<\/jats:sup>, Co<jats:sup>2+<\/jats:sup>, Fe<jats:sup>2+<\/jats:sup>, and Ni<jats:sup>2+<\/jats:sup> were observed at 55, 65, 110, 125, and 284\u2002\u03bcM, respectively. Kinetic analyses indicate that the metal ions are acting as competitive inhibitors of CK-1 with respect to the protein substrate (casein) and as noncompetitive inhibitors with respect to the nucleotide substrate (ATP). The inhibition of CK-1 by the different metal ions can be reversed by EGTA. <\/jats:p>","DOI":"10.1139\/o88-031","type":"journal-article","created":{"date-parts":[[2009,12,23]],"date-time":"2009-12-23T19:36:00Z","timestamp":1261596960000},"page":"238-243","source":"Crossref","is-referenced-by-count":4,"title":["Inhibition of glycogen synthase (casein) kinase-1 by divalent metal ions"],"prefix":"10.1139","volume":"66","author":[{"given":"Toolsee J.","family":"Singh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Biochemistry and Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdnsciencepub.com\/doi\/pdf\/10.1139\/o88-031","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T18:53:19Z","timestamp":1751482399000},"score":1,"resource":{"primary":{"URL":"https:\/\/cdnsciencepub.com\/doi\/10.1139\/o88-031"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1988,3,1]]}},"alternative-id":["10.1139\/o88-031"],"URL":"https:\/\/doi.org\/10.1139\/o88-031","relation":{},"ISSN":["0829-8211","1208-6002"],"issn-type":[{"type":"print","value":"0829-8211"},{"type":"electronic","value":"1208-6002"}],"subject":[],"published":{"date-parts":[[1988,3,1]]}}}