{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T11:47:00Z","timestamp":1759664820215,"version":"3.41.0"},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"5","license":[{"start":{"date-parts":[[1988,5,1]],"date-time":"1988-05-01T00:00:00Z","timestamp":578448000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. Cell Biol."],"published-print":{"date-parts":[[1988,5,1]]},"abstract":"<jats:p> The identity of a dithiol (designated DF<jats:sub>B<\/jats:sub>) of relative mass (M<jats:sub>r<\/jats:sub>)\u2002=\u200213\u2002000, reported previously to be present infraction B of rat liver cytosol and to participate as a cofactor in the 5\u2032-deiodination of iodothyronines, has been investigated. Substitution of highly purified thioredoxin from Escherichia coli for fraction B or of highly purified thioredoxin reductase from either E. coli or rat liver for cytosolic fraction A (containing DF<jats:sub>B<\/jats:sub> reductase) permits deiodination of 3,3\u2032,5\u2032-[<jats:sup>l25<\/jats:sup>I]triiodothyronine by rat liver microsomes to proceed. Addition of antibodies to highly purified rat-liver thioredoxin or thioredoxin reductase inhibits deiodination. Thus, the thioredoxin system largely accounts for the activity of the cytosolic cofactor system supporting 5\u2032-deiodination of 3,3\u2032,5\u2032-triiodothyronine in rat liver. <\/jats:p>","DOI":"10.1139\/o88-057","type":"journal-article","created":{"date-parts":[[2009,12,23]],"date-time":"2009-12-23T19:36:11Z","timestamp":1261596971000},"page":"460-464","source":"Crossref","is-referenced-by-count":7,"title":["Bacterial and mammalian thioredoxin systems activate iodothyronine 5\u2032-deiodination"],"prefix":"10.1139","volume":"66","author":[{"given":"Arun K.","family":"Das","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian C. W.","family":"Hummel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florence K.","family":"Gleason","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arne","family":"Holmgren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul G.","family":"Walfish","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Biochemistry and Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdnsciencepub.com\/doi\/pdf\/10.1139\/o88-057","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T18:11:27Z","timestamp":1751479887000},"score":1,"resource":{"primary":{"URL":"https:\/\/cdnsciencepub.com\/doi\/10.1139\/o88-057"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1988,5,1]]}},"alternative-id":["10.1139\/o88-057"],"URL":"https:\/\/doi.org\/10.1139\/o88-057","relation":{},"ISSN":["0829-8211","1208-6002"],"issn-type":[{"type":"print","value":"0829-8211"},{"type":"electronic","value":"1208-6002"}],"subject":[],"published":{"date-parts":[[1988,5,1]]}}}