{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:59:23Z","timestamp":1773291563546,"version":"3.50.1"},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"4-5","license":[{"start":{"date-parts":[[1989,4,1]],"date-time":"1989-04-01T00:00:00Z","timestamp":607392000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochem. Cell Biol."],"published-print":{"date-parts":[[1989,4,1]]},"abstract":"<jats:p> Equilibria and kinetics of cyanide binding to canine myeloperoxidase were studied. Spectral results support the presence of two heme binding sites; an isosbestic point at 444\u2002nm and a linear Scatchard plot suggest that the binding affinity of cyanide to the two subunits of the enzyme is the same. The dissociation constant is 0.53\u2002\u03bcM. The pH dependence of the apparent second order rate constant indicates the presence of an acid\u2013base group on the enzyme with a pK<jats:sub>a<\/jats:sub> of 3.8\u2002\u00b1\u20020.1. The protonated form of cyanide binds to the basic enzyme with a rate constant of (4.3\u2002\u00b1\u20020.3)\u2002\u00d7\u200210<jats:sup>6<\/jats:sup>\u2002M<jats:sup>\u22121<\/jats:sup>\u2002s<jats:sup>\u22121<\/jats:sup>.Key words: myeloperoxidase, cyanide binding, equilibrium binding, ligand binding kinetics. <\/jats:p>","DOI":"10.1139\/o89-029","type":"journal-article","created":{"date-parts":[[2009,12,23]],"date-time":"2009-12-23T19:37:04Z","timestamp":1261597024000},"page":"187-191","source":"Crossref","is-referenced-by-count":5,"title":["Cyanide binding to canine myeloperoxidase"],"prefix":"10.1139","volume":"67","author":[{"given":"Leah A.","family":"Marquez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. Brian","family":"Dunford","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Biochemistry and Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdnsciencepub.com\/doi\/pdf\/10.1139\/o89-029","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T18:51:54Z","timestamp":1751482314000},"score":1,"resource":{"primary":{"URL":"https:\/\/cdnsciencepub.com\/doi\/10.1139\/o89-029"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,4,1]]},"references-count":0,"journal-issue":{"issue":"4-5","published-print":{"date-parts":[[1989,4,1]]}},"alternative-id":["10.1139\/o89-029"],"URL":"https:\/\/doi.org\/10.1139\/o89-029","relation":{},"ISSN":["0829-8211","1208-6002"],"issn-type":[{"value":"0829-8211","type":"print"},{"value":"1208-6002","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,4,1]]}}}