{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:00:21Z","timestamp":1790823621039,"version":"4.1.0"},"reference-count":10,"publisher":"Canadian Science Publishing","issue":"4","license":[{"start":{"date-parts":[[2004,4,1]],"date-time":"2004-04-01T00:00:00Z","timestamp":1080777600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2004,4,1]]},"abstract":"<jats:p> Escherichia coli O14:K7 is a rough strain, lacking a typical O antigen, in which the enterobacterial common antigen is attached to the lipopolysaccharide core. The rough phenotype was previously mapped to the O antigen gene cluster; however, the nature of the nonfunctional locus was not defined. In this study, we have shown that the O antigen gene cluster of an O14:K7 type strain (Su4411\/41) was most likely deleted via homologous recombination between the GDP\u2013mannose pathway genes (manB and manC) of the colanic acid and O antigen gene clusters. A similar recombination event has previously been inferred for the deletion of E. coli Sonnei chromosomal O antigen genes. Therefore, recombination between the GDP\u2013mannose pathway genes provides a convenient mechanism for the deletion of O antigen genes, which may occur if the typical O antigen becomes redundant.Key words: colanic acid, enterobacterial common antigen, GDP\u2013mannose pathway, O14:K7, O antigen. <\/jats:p>","DOI":"10.1139\/w04-010","type":"journal-article","created":{"date-parts":[[2004,6,22]],"date-time":"2004-06-22T13:55:32Z","timestamp":1087912532000},"page":"299-302","source":"Crossref","is-referenced-by-count":11,"title":["Deletion of the <i>Escherichia coli<\/i> O14:K7 O antigen gene cluster"],"prefix":"10.1139","volume":"50","author":[{"given":"Slade O","family":"Jensen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter R","family":"Reeves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"p_1\/p_1_1","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1046\/j.1365-2958.1997.6121983.x","volume":"26","author":"Espeli O.","year":"1997","journal-title":"Mol. 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