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The efficacy of the vaccine was compared with that of an intramuscular vaccine that was formulated with the same immunogen plus Freund's adjuvant. Our results show that serum immunoglobulin G titers of vaccinated animals were similarly enhanced in both cases. However, the intranasal vaccine elicited higher vaginal-wash-specific immunoglobulin A titers against rFimHt than the intramuscular route. Both vaccines reduced the in vivo colonization of the bladder by uropathogenic Escherichia coli more than 100-fold in a murine cystitis model. Our results indicate that a recombinant truncated FimH adhesin plus CpG oligodeoxynucleotides is a suitable immunogenic combination that can contribute to the development of a highly efficacious urinary tract infection vaccine.Key words: FimH adhesin, CpG oligodeoxynucleotides, intranasal vaccine.<\/jats:p>","DOI":"10.1139\/w06-065","type":"journal-article","created":{"date-parts":[[2007,1,10]],"date-time":"2007-01-10T21:00:33Z","timestamp":1168462833000},"page":"1093-1102","source":"Crossref","is-referenced-by-count":47,"title":["Intranasal immunization with a recombinant truncated FimH adhesin adjuvanted with CpG oligodeoxynucleotides protects mice against uropathogenic<i>Escherichia coli<\/i>challenge"],"prefix":"10.1139","volume":"52","author":[{"given":"Thelma Ver\u00f3nica","family":"Poggio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Leonardo","family":"La Torre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo Alberto","family":"Scodeller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"issue":"12","key":"p_1\/p_1_1","doi-asserted-by":"crossref","first-page":"5530","DOI":"10.1128\/JB.169.12.5530-5536.1987","volume":"169","author":"Abraham S.N.","year":"1987","journal-title":"J. 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