{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T10:22:20Z","timestamp":1758709340191},"reference-count":34,"publisher":"Canadian Science Publishing","issue":"5","license":[{"start":{"date-parts":[[2007,5,1]],"date-time":"2007-05-01T00:00:00Z","timestamp":1177977600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:p>An optimized cell culture\u00a0immunofluorescence (IFA) procedure, using the HCT-8 cell line, was evaluated in blind trials to determine the sensitivity and reproducibility of measuring the infectivity of flow-cytometry-prepared inocula of Cryptosporidium parvum oocysts. In separate trials, suspensions consisting of between 0% and 100% viable oocysts were prepared at the US Environmental Protection Agency, shipped to the American Water Laboratory, and analyzed blindly by cell culture IFA. Data indicated the control (100% live) oocyst suspensions yielded statistically similar results to cell culture dose\u2013response curve data developed previously at the American Water Laboratory. For test samples containing oocyst suspensions of unknown infectivity, cell culture IFA analyses indicated a high degree of correlation (r<jats:sup>2<\/jats:sup>= 0.89; n\u00a0= 26) with the values expected by the US Environmental Protection Agency. Cell culture infectivity correlates well with neonatal mouse infectivity assays, and these blind validation trials provide credibility for the cell culture IFA procedure as a cost-effective and expedient alternative to mouse infectivity assays for determining in vitro infectivity of C.\u00a0parvum oocysts.<\/jats:p>","DOI":"10.1139\/w07-032","type":"journal-article","created":{"date-parts":[[2007,7,31]],"date-time":"2007-07-31T18:30:31Z","timestamp":1185906631000},"page":"656-663","source":"Crossref","is-referenced-by-count":9,"title":["Blind trials evaluating in vitro infectivity of<i>Cryptosporidium<\/i>oocysts using cell culture immunofluorescence"],"prefix":"10.1139","volume":"53","author":[{"given":"Zia","family":"Bukhari","sequence":"first","affiliation":[{"name":"American Water, 1025 Laurel Oak Road, Voorhees, NJ 08043, USA."},{"name":"Thames Water, Spencer House, Manor Farm Road, Reading, UK RG2 OJN."},{"name":"United States Environmental Protection Agency, 26 West Martin Luther King Drive, Cincinnati, OH 45268-1320, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David\u00a0M.","family":"Holt","sequence":"additional","affiliation":[{"name":"American Water, 1025 Laurel Oak Road, Voorhees, NJ 08043, USA."},{"name":"Thames Water, Spencer House, Manor Farm Road, Reading, UK RG2 OJN."},{"name":"United States Environmental Protection Agency, 26 West Martin Luther King Drive, Cincinnati, OH 45268-1320, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael\u00a0W.","family":"Ware","sequence":"additional","affiliation":[{"name":"American Water, 1025 Laurel Oak Road, Voorhees, NJ 08043, USA."},{"name":"Thames Water, Spencer House, Manor Farm Road, Reading, UK RG2 OJN."},{"name":"United States Environmental Protection Agency, 26 West Martin Luther King Drive, Cincinnati, OH 45268-1320, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank\u00a0W.","family":"Schaefer","sequence":"additional","affiliation":[{"name":"American Water, 1025 Laurel Oak Road, Voorhees, NJ 08043, USA."},{"name":"Thames Water, Spencer House, Manor Farm Road, Reading, UK RG2 OJN."},{"name":"United States Environmental Protection Agency, 26 West Martin Luther King Drive, Cincinnati, OH 45268-1320, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"atypb1\/ref1","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1002\/j.1551-8833.2004.tb10706.x","volume":"96","author":"Aboytes R.","year":"2004","journal-title":"J Am Water Works Assoc."},{"key":"atypb2\/ref2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.10.5670-5675.2002"},{"key":"atypb3\/ref3","first-page":"89S. 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