{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T19:43:36Z","timestamp":1770752616589,"version":"3.50.0"},"reference-count":38,"publisher":"Canadian Science Publishing","issue":"8","license":[{"start":{"date-parts":[[2007,8,1]],"date-time":"2007-08-01T00:00:00Z","timestamp":1185926400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:p>We characterized a population of Enterobacter spp. of the Enterobacter cloacae complex isolated from an oligotrophic lake; most isolates were identified as E.\u00a0cloacae. Fingerprinting polymerase chain reaction (PCR), along with morphological, biochemical, physiological, and plasmid profiles analyses, including antimicrobial susceptibility testing, were performed on 22 environmental isolates. Misidentification occurred when using the API 20E identification system. Analysis of 16S rDNA sequences confirmed the close relatedness between species of the E.\u00a0cloacae complex. The tDNA PCR allowed the differentiation and identification of the E.\u00a0cloacae isolates. Evaluation of genetic diversity by 16S rDNA sequence, tDNA, internal transcribed spacers, and enterobacterial repetitive intergenic concensus profiles revealed nearly identical isolates, although they exhibited different physiological and antimicrobial resistance profiles. Among the Enterobacter isolates, 96% were resistant to at least one antimicrobial; multiple resistance was also found at a high frequency (86%). The antimicrobials against which resistance was found most frequently were \u03b2-lactams, chloramphenicol, and streptomycin. Plasmids were found in 21 of the 22 Enterobacter isolates. This confirms the conception that antibiotic resistance can occur in oligotrophic freshwater lake bacteria, which has important implications for public health.<\/jats:p>","DOI":"10.1139\/w07-060","type":"journal-article","created":{"date-parts":[[2007,9,26]],"date-time":"2007-09-26T17:44:00Z","timestamp":1190828640000},"page":"983-991","source":"Crossref","is-referenced-by-count":4,"title":["Phenotypic and genetic analysis of Enterobacter spp. from a Brazilian oligotrophic freshwater lake"],"prefix":"10.1139","volume":"53","author":[{"given":"Daniela Santos","family":"Pontes","sequence":"first","affiliation":[{"name":"Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Av. Ant\u00f4nio Carlos, 6627, Belo Horizonte-MG, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udia Iracema","family":"Lima-Bittencourt","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Av. Ant\u00f4nio Carlos, 6627, Belo Horizonte-MG, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcela\u00a0Santiago\u00a0Pacheco","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Av. Ant\u00f4nio Carlos, 6627, Belo Horizonte-MG, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edmar","family":"Chartone-Souza","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Av. Ant\u00f4nio Carlos, 6627, Belo Horizonte-MG, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9a\u00a0Maria\u00a0Amaral","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Av. Ant\u00f4nio Carlos, 6627, Belo Horizonte-MG, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"atypb1\/ref1","doi-asserted-by":"publisher","DOI":"10.1139\/w05-022"},{"key":"atypb2\/ref2","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1128\/JCM.23.6.1114-1120.1986","volume":"23","author":"Brenner D.J.","year":"1986","journal-title":"J. Clin. Microbiol."},{"key":"atypb3\/ref3","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0378-1097(02)00559-1","volume":"209","author":"Br\u00fcckner R.","year":"2002","journal-title":"FEMS Microbiol. Lett."},{"key":"atypb4\/ref4","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.11.3865-3870.2001"},{"key":"atypb5\/ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.02.003"},{"key":"atypb6\/ref6","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1089\/ind.2006.2.51","volume":"2","author":"Denery J.R.","year":"2006","journal-title":"Ind. Biotechnol."},{"key":"atypb7\/ref7","first-page":"167","volume":"119","author":"Dolzani L.","year":"1994","journal-title":"FEMS Microbiol. Lett."},{"key":"atypb8\/ref8","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1101\/gr.8.3.186","volume":"8","author":"Ewing B.","year":"1998","journal-title":"Genome Res."},{"key":"atypb9\/ref9","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1053\/jhin.2001.1053","volume":"49","author":"Fernandez-Baca V.","year":"2001","journal-title":"J. Hosp. Infect."},{"key":"atypb10\/ref10","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1101\/gr.8.3.195","volume":"8","author":"Gordon D.","year":"1998","journal-title":"Genome Res."},{"key":"atypb12\/ref12","first-page":"1","volume":"4","author":"Hammer \u00d8.","year":"2001","journal-title":"Palaeont. Electr."},{"key":"atypb13\/ref13","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.9.5306-5318.2003"},{"key":"atypb14\/ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2004.12.009"},{"key":"atypb15\/ref15","first-page":"6","volume":"26","author":"Iversen C.","year":"2006","journal-title":"BMC Microbiol."},{"key":"atypb16\/ref16","doi-asserted-by":"crossref","first-page":"1846","DOI":"10.1128\/JCM.36.7.1846-1852.1998","volume":"36","author":"Jalaluddin S.","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"atypb17\/ref17","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.8.4831-4839.2004"},{"key":"atypb18\/ref18","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1128\/JB.145.3.1365-1373.1981","volume":"145","author":"Kado C.I.","year":"1981","journal-title":"J. Bacteriol."},{"key":"atypb19\/ref19","doi-asserted-by":"publisher","DOI":"10.1007\/BF01731581"},{"key":"atypb20\/ref20","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/5.2.150"},{"key":"atypb21\/ref21","doi-asserted-by":"crossref","first-page":"3614","DOI":"10.1128\/AEM.63.9.3614-3621.1997","volume":"63","author":"Kuske C.R.","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"atypb22\/ref22","doi-asserted-by":"crossref","first-page":"2076","DOI":"10.1128\/JCM.38.6.2076-2080.2000","volume":"38","author":"Lu J.J.","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"atypb23\/ref23","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1111\/j.1574-6941.2001.tb00786.x","volume":"35","author":"McCaig A.E.","year":"2001","journal-title":"FEMS Microbiol. Ecol."},{"key":"atypb24\/ref24","doi-asserted-by":"publisher","DOI":"10.1016\/0043-1354(95)00013-B"},{"key":"atypb25\/ref25","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(99)80076-X"},{"key":"atypb27\/ref27","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1590\/1808-1657v70p3672003","volume":"70","author":"Neto J.R.","year":"2003","journal-title":"Arq. Inst. Biol. (Sao Paulo)"},{"key":"atypb28\/ref28","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1128\/AEM.45.1.79-83.1983","volume":"45","author":"Niemi M.","year":"1983","journal-title":"Appl. Environ. Microbiol."},{"key":"atypb29\/ref29","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s00253-002-1202-6","volume":"61","author":"Peng L.","year":"2003","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"atypb30\/ref30","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-007-0219-3"},{"key":"atypb31\/ref31","first-page":"406","volume":"4","author":"Saitou N.","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"atypb32\/ref32","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1111\/j.1574-6941.2003.tb01073.x","volume":"43","author":"Schwartz T.","year":"2003","journal-title":"FEMS Microbiol. Ecol."},{"key":"atypb33\/ref33","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/S0924-8579(01)00463-0","volume":"18","author":"Stock I.","year":"2001","journal-title":"Int. J. Antimicrob. Agents"},{"key":"atypb34\/ref34","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2005.04.003"},{"key":"atypb35\/ref35","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2004.01.023"},{"key":"atypb36\/ref36","doi-asserted-by":"crossref","first-page":"3674","DOI":"10.1128\/JCM.36.12.3674-3679.1998","volume":"36","author":"Tang Y.W.","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"atypb37\/ref37","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.24.6823"},{"key":"atypb38\/ref38","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(01)00220-2"},{"key":"atypb39\/ref39","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.4.861"},{"key":"atypb40\/ref40","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0924-8579(97)00046-0","volume":"9","author":"Zaher A.","year":"1998","journal-title":"Int. J. Antimicrob. Agents"}],"container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/full-xml\/10.1139\/W07-060","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/W07-060","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T06:03:06Z","timestamp":1737439386000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/W07-060"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8]]},"references-count":38,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2007,8]]}},"alternative-id":["10.1139\/W07-060"],"URL":"https:\/\/doi.org\/10.1139\/w07-060","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8]]}}}