{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T21:05:49Z","timestamp":1764363949579},"reference-count":15,"publisher":"Canadian Science Publishing","issue":"9","license":[{"start":{"date-parts":[[2007,9,1]],"date-time":"2007-09-01T00:00:00Z","timestamp":1188604800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:p> Cyanide or metal cyanide contaminations have become serious environmental and food-health problems. A fungal mutant of Trichoderma koningii , TkA8, constructed by restriction enzyme-mediated integration, has been verified to have a high cyanide degradation ability in our previous study. In this study, the mutant cells were entrapped in sodium-alginate (Na-alginate) immobilization beads to degrade cyanide and ferrocyanide in a liquid mineral medium. The results showed that the fungus in immobilization beads consisting of 3% Na-alginate and 3% CaCl<jats:sub>2<\/jats:sub> could degrade cyanide more efficiently than a nonimmobilized fungal culture. For maximum degradation efficiency, the optimal ratio of Na-alginate and wet fungal biomass was 20:1 (m\/m) and the initial pH was 6.5. In comparison, cell immobilization took at least 3 and 8 days earlier, respectively, to completely degrade cyanide and ferrocyanide. In addition, we showed that the immobilized beads could be easily recovered from the medium and reused for up to 5 batches without significant losses of fungal remediation abilities. The results of this study provide a promising alternative method for the large-scale remediation of soil or water systems from cyanide contamination. <\/jats:p>","DOI":"10.1139\/w07-070","type":"journal-article","created":{"date-parts":[[2008,4,14]],"date-time":"2008-04-14T19:17:33Z","timestamp":1208200653000},"page":"1033-1037","source":"Crossref","is-referenced-by-count":19,"title":["Efficient biodegradation of cyanide and ferrocyanide by Na-alginate beads immobilized with fungal cells of <i>Trichoderma koningii<\/i>"],"prefix":"10.1139","volume":"53","author":[{"given":"Xiaoying","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Plant Science, School of Agriculture and Biology, Shanghai Jiaotong University, 2678 Qixin Road Shanghai, 201101 People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lixing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Plant Science, School of Agriculture and Biology, Shanghai Jiaotong University, 2678 Qixin Road Shanghai, 201101 People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunpeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Plant Science, School of Agriculture and Biology, Shanghai Jiaotong University, 2678 Qixin Road Shanghai, 201101 People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shufa","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Plant Science, School of Agriculture and Biology, Shanghai Jiaotong University, 2678 Qixin Road Shanghai, 201101 People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Plant Science, School of Agriculture and Biology, Shanghai Jiaotong University, 2678 Qixin Road Shanghai, 201101 People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"atypb1\/ref1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/S0141-0229(98)00055-6","volume":"23","author":"Barcley M.","year":"1998","journal-title":"Enzyme Microb. 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