{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T13:23:47Z","timestamp":1773149027408,"version":"3.50.1"},"reference-count":23,"publisher":"Canadian Science Publishing","issue":"11","license":[{"start":{"date-parts":[[2007,11,1]],"date-time":"2007-11-01T00:00:00Z","timestamp":1193875200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:p>Serratia marcescens is a nosocomial agent with a natural resistance to a broad spectrum of antibiotics, making the treatment of its infections very challenging. This study examines the influence of salicylate, sucrose, temperature, and pH variability on membrane permeability and susceptibility of S.\u00a0marcescens to norfloxacin (hydrophilic fluoroquinolone) and nalidixic acid (hydrophobic quinolone). Resistance of wild-type S.\u00a0marcescens UOC-67 (ATCC 13880) to norfloxacin and nalidixic acid was assessed by minimal inhibitory concentration (MIC) assays after growth in the presence of various concentrations of sucrose and salicylate and different temperatures and pH values. Norfloxacin and nalidixic acid accumulation was determined in the absence and presence of (i) carbonyl cyanide m-chlorophenylhydrazone (CCCP), a proton-motive-force collapser, and (ii) Phe-Arg \u03b2-naphthylamide (PA\u03b2N), an efflux pump inhibitor. Accumulation of norfloxacin decreased when S.\u00a0marcescens was grown in high concentrations of salicylate (8\u00a0mmol\/L) and sucrose (10% m\/v), at high temperature (42\u00a0\u00b0C), and at pH\u00a06, and it was restored in the presence of CCCP because of the collapse of proton-gradient-dependent efflux in S.\u00a0marcescens. Although nalidixic acid accumulation was observed, it was not affected by salicylate, sucrose, pH, or temperature changes. In the absence of PA\u03b2N, and either in the presence or absence of CCCP, a plateau was reached in the nalidixic acid accumulation for all environmental conditions. With the addition of 20\u00a0mg\/L PA\u03b2N nalidixic acid accumulation is restored for all environmental conditions, suggesting that this quinolone is recognized by a yet to be identified S.\u00a0marcescens pump that does not use proton motive force as its energy source.<\/jats:p>","DOI":"10.1139\/w07-097","type":"journal-article","created":{"date-parts":[[2007,11,16]],"date-time":"2007-11-16T17:19:40Z","timestamp":1195233580000},"page":"1239-1245","source":"Crossref","is-referenced-by-count":15,"title":["Fluoroquinolone resistance of<i>Serratia marcescens<\/i>: sucrose, salicylate, temperature, and pH induction of phenotypic resistance"],"prefix":"10.1139","volume":"53","author":[{"given":"Sanela","family":"Begic","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Manitoba, Winnipeg, MB R3T\u00a02N2, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth A.","family":"Worobec","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Manitoba, Winnipeg, MB R3T\u00a02N2, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"atypb1\/ref1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28428-0"},{"key":"atypb2\/ref2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/46.2.279"},{"key":"atypb3\/ref3","doi-asserted-by":"publisher","DOI":"10.1139\/cjm-46-8-716"},{"key":"atypb5\/ref5","doi-asserted-by":"crossref","first-page":"7856","DOI":"10.1128\/JB.175.24.7856-7862.1993","volume":"175","author":"Cohen S.P.","year":"1993","journal-title":"J. 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