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Based on the variability of the A36R gene, we describe a simple 20\u00a0min PCR assay followed by a 1\u00a0h digest with 3 different restriction enzymes. This assay enables rapid discrimination between Cowpox virus and Monkeypox virus and discrimination of the most prevalent members of the Vaccinia virus and Camelpox virus. The test was orthopoxvirus specificand did not react with parapox (Orf) virus. Moreover, the amplified fragments were also well suited for additional genotyping by direct DNA sequencing. <\/jats:p>","DOI":"10.1139\/w07-129","type":"journal-article","created":{"date-parts":[[2008,4,3]],"date-time":"2008-04-03T15:17:37Z","timestamp":1207235857000},"page":"159-162","source":"Crossref","is-referenced-by-count":5,"title":["A simple restriction fragment PCR approach for discrimination of humanpathogenic Old World animal <i>Orthopoxvirus<\/i> species"],"prefix":"10.1139","volume":"54","author":[{"given":"Hartwig P.","family":"Huemer","sequence":"first","affiliation":[{"name":"Innsbruck Medical University, Department of Hygiene, Microbiology and Social Medicine, Fritz-Pregl-Strasse 3, A-6020 Innsbruck, Austria."},{"name":"Innsbruck Medical University, Department of Dermatology and Venerology, Anichstra\u00dfe 35, \rA-6020 Innsbruck, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bettina","family":"H\u00f6nlinger","sequence":"additional","affiliation":[{"name":"Innsbruck Medical University, Department of Hygiene, Microbiology and Social Medicine, Fritz-Pregl-Strasse 3, A-6020 Innsbruck, Austria."},{"name":"Innsbruck Medical University, Department of Dermatology and Venerology, Anichstra\u00dfe 35, \rA-6020 Innsbruck, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhard","family":"H\u00f6pfl","sequence":"additional","affiliation":[{"name":"Innsbruck Medical University, Department of Hygiene, Microbiology and Social Medicine, Fritz-Pregl-Strasse 3, A-6020 Innsbruck, Austria."},{"name":"Innsbruck Medical University, Department of Dermatology and Venerology, Anichstra\u00dfe 35, \rA-6020 Innsbruck, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"atypb1\/ref1","first-page":"193","volume":"31","author":"Chernos V.I.","year":"1987","journal-title":"Acta Virol."},{"key":"atypb2\/ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.07.012"},{"key":"atypb3\/ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)92387-W"},{"key":"atypb4\/ref4","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81090-0"},{"key":"atypb5\/ref5","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81260-0"},{"key":"atypb6\/ref6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2005.06731.x"},{"key":"atypb7\/ref7","first-page":"29","volume":"27","author":"Jezek Z.","year":"1983","journal-title":"J. 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