{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T23:02:13Z","timestamp":1762297333298},"reference-count":33,"publisher":"Canadian Science Publishing","issue":"3","license":[{"start":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T00:00:00Z","timestamp":1204329600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:p>We described 2 unusual Neisseria meningitidis strains isolated from epidemiologically unrelated invasive meningococcal disease cases in Ontario, Canada. Both isolates have features typical of serogroup Y N.\u00a0meningitidis: are of serotype 2c, are of the multi-locus sequence types typical of the serogroup Y strains in Canada, and are genotyped as serogroup Y based on a previously described PCR\u2013ELISA method that detects the serogroup-Y-specific siaD gene. However, both strains were poly-agglutinable in both anti-Y and anti-W135 antisera. Further studies on 1 of these 2 isolates showed the presence of glucose and galactose as well as sialic acids in its purified capsular polysaccharide, suggesting the presence of both serogroup Y and serogroup W135 polysaccharides. Rabbit antisera produced to this strain contained antibodies to both purified serogroup Y and serogroup W135 capsular polysaccharides. Absorption experiments with either serogroup Y or serogroup W135 bacteria confirmed the presence of antibodies to these 2 different polysaccharides. DNA sequencing of the cps operon from both isolates revealed a siaD gene with 99.7% homology to the published siaD sequence from a serogroup Y strain but with 3 point mutations that all resulted in amino acid changes. How these strains may affect results of routine surveillance, PCR diagnosis, and immuno-protection by vaccination are discussed.<\/jats:p>","DOI":"10.1139\/w07-132","type":"journal-article","created":{"date-parts":[[2008,4,3]],"date-time":"2008-04-03T19:17:52Z","timestamp":1207250272000},"page":"229-234","source":"Crossref","is-referenced-by-count":19,"title":["Immunochemical studies and genetic background of two<i>Neisseria meningitidis<\/i>isolates expressing unusual capsule polysaccharide antigens with specificities of both serogroup Y and W135"],"prefix":"10.1139","volume":"54","author":[{"given":"Raymond S.W.","family":"Tsang","sequence":"first","affiliation":[{"name":"Laboratory for Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E\u00a03R2, Canada."},{"name":"DNA Core Facility, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E\u00a03R2, Canada."},{"name":"Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA."},{"name":"Walter Reed Army Institute of Research, Department of Bacterial Diseases, Silver Spring, MD 20910, USA."},{"name":"Ontario Public Health Laboratory, Ministry of Health and Long Term Care, 81 Resources Road, Etobicoke, Toronto, ON M9P\u00a03T1, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao Ming","family":"Tsai","sequence":"additional","affiliation":[{"name":"Laboratory for Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E\u00a03R2, Canada."},{"name":"DNA Core Facility, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E\u00a03R2, Canada."},{"name":"Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA."},{"name":"Walter Reed Army Institute of Research, Department of Bacterial Diseases, Silver Spring, MD 20910, USA."},{"name":"Ontario Public Health Laboratory, Ministry of Health and Long Term Care, 81 Resources Road, Etobicoke, Toronto, ON M9P\u00a03T1, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Averil M.","family":"Henderson","sequence":"additional","affiliation":[{"name":"Laboratory for Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E\u00a03R2, Canada."},{"name":"DNA Core Facility, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E\u00a03R2, Canada."},{"name":"Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA."},{"name":"Walter Reed Army Institute of Research, Department of Bacterial Diseases, Silver Spring, MD 20910, USA."},{"name":"Ontario Public Health Laboratory, Ministry of Health and Long Term Care, 81 Resources Road, Etobicoke, Toronto, ON M9P\u00a03T1, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaun","family":"Tyler","sequence":"additional","affiliation":[{"name":"Laboratory for Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E\u00a03R2, Canada."},{"name":"DNA Core Facility, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E\u00a03R2, Canada."},{"name":"Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA."},{"name":"Walter Reed Army Institute of Research, Department of Bacterial Diseases, Silver Spring, MD 20910, USA."},{"name":"Ontario Public Health Laboratory, Ministry of Health and Long Term Care, 81 Resources Road, Etobicoke, Toronto, ON M9P\u00a03T1, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis K.S.","family":"Law","sequence":"additional","affiliation":[{"name":"Laboratory for Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E\u00a03R2, Canada."},{"name":"DNA Core Facility, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E\u00a03R2, Canada."},{"name":"Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA."},{"name":"Walter Reed Army Institute of Research, Department of Bacterial Diseases, Silver Spring, MD 20910, USA."},{"name":"Ontario Public Health Laboratory, Ministry of Health 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