{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:24:34Z","timestamp":1790904274576,"version":"4.1.0"},"reference-count":21,"publisher":"Canadian Science Publishing","issue":"4","license":[{"start":{"date-parts":[[2008,4,1]],"date-time":"2008-04-01T00:00:00Z","timestamp":1207008000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:p> Viral and bacterial associations appear to be implicated in the development of periodontal infections. Little information is available describing the periodontopathic agents in root canals with necrotic pulp. In this study, the occurrence and the combinations among herpes simplex virus type 1 (HSV-1) and Dialister pneumosintes , Tannerella forsythia , and Treponema denticola in patients with chronic periodontitis and necrotic pulp were evaluated. Clinical samples from healthy subjects and patients with periodontal or pulp infections were analyzed using a nested polymerase chain reaction PCR to detect HSV and PCR to detect the 3 periodontal bacteria. The presence of Tannerella forsythia and Treponema denticola was observed in healthy, periodontitis, and necrotic pulp patients. HSV was observed in periodontitis and necrotic pulp patients, and no healthy subject harbored D. pneumosintes or HSV. The occurrence of Tannerella forsythia was not statistically significant in patients with necrotic pulp (P = 0.704). Periodontal bacteria were observed varying from 10.3% to 20.7% in periodontitis and necrotic pulp patients. The presence of Treponema denticola\u00a0\u2013 HSV association was predominant in patients showing necrotic pulp (24.1%); however, HSV alone was observed in one patient with periodontitis and in another patient with necrotic pulp. The presence of double association among bacteria or bacteria\u00a0\u2013 HSV could indicate a role in both periodontitis and necrotic pulp, and Tannerella forsythia\u00a0\u2013 Treponema denticola\u00a0\u2013 HSV and Tannerella forsythia\u00a0\u2013 D.\u00a0pneumosintes\u00a0\u2013 Treponema denticola\u00a0\u2013 HSV associations might be important in periodontitis. <\/jats:p>","DOI":"10.1139\/w08-006","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T15:57:09Z","timestamp":1265212629000},"page":"326-330","source":"Crossref","is-referenced-by-count":25,"title":["Occurrence of herpes simplex virus 1 and three periodontal bacteria in patients with chronic periodontitis and necrotic pulp"],"prefix":"10.1139","volume":"54","author":[{"given":"Sheila Alexandra Belini","family":"Nishiyama","sequence":"first","affiliation":[{"name":"Department of Microbiology, Institute of Biomedical Science, University of S\u00e3o Paulo, Av. 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