{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:18:40Z","timestamp":1791303520108,"version":"4.1.0"},"reference-count":48,"publisher":"Canadian Science Publishing","issue":"12","license":[{"start":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T00:00:00Z","timestamp":1228089600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:p>In the last decade, Escherichia coli O157:H7 have emerged as important pathogens of the gastrointestinal tract of humans. Healthy cattle have been identified as the primary reservoir, however, the factors affecting heterogeneous E. coli O157:H7 fecal shedding are not fully understood. The aim of this study was to investigate the contribution of E. coli O157:H7 colonization of small and large intestinal sites to the heterogeneity of fecal shedding in cattle. There was a dose-dependant E. coli O157:H7 E318N colonization of duodenum, jejunum, ileum, cecum, ascending colon, spiral colon, descending colon, and the rectoanal junction in vitro with no difference in E. coli O157:H7 colonization of the rectoanal junction and other intestinal sites. There were 10\u2013100 times greater E. coli O157:H7 colonization of intestinal sites from persistent shedding cattle compared with nonpersistent shedding cattle. Novel pathologies were associated with E. coli O157:H7 colonization sites in the small and large intestine. The first pathology, focal petechiae, was present throughout the intestinal tract of cattle that ceased shedding E. coli O157:H7 for 5\u201312\u00a0weeks or in the jejunum, ileum, cecum, and ascending colon of cattle shedding E. coli O157:H7 for 4\u20135\u00a0months. The second pathology, mucosal hemorrhages, was present in the same sites as the focal petechiae in cattle shedding for 5\u00a0months and these hemorrhages were in the final stages of repair. Several features of these hemorrhages support this conclusion including the brown appearance, low amount of classic E. coli O157:H7 induced A\/E lesions, flattened epithelium, and blunted villi. Although mucosal hemorrhages were present in the jejunum, ileum, cecum, and ascending colon in cattle shedding for 4\u00a0months, many other pathologies were also present that were indicative of hemorrhagic enteritis as evidenced by the blood red appearance of hemorrhages, severe edema, and dark red erythema. Escherichia coli O157:H7 were associated with both pathologies suggesting it is the causative agent. The current study supports a relationship between the amount of E. coli O157:H7 colonization in intestinal sites and heterogeneous fecal shedding by cattle.<\/jats:p>","DOI":"10.1139\/w08-090","type":"journal-article","created":{"date-parts":[[2008,12,18]],"date-time":"2008-12-18T16:56:17Z","timestamp":1229619377000},"page":"984-995","source":"Crossref","is-referenced-by-count":31,"title":["Heterogeneity in enterohemorrhagic<i>Escherichia coli<\/i>O157:H7 fecal shedding in cattle is related to<i>Escherichia coli<\/i>O157:H7 colonization of the small and large intestine"],"prefix":"10.1139","volume":"54","author":[{"given":"Danica","family":"Baines","sequence":"first","affiliation":[{"name":"Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB\u00a0T1J\u00a04B1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byron","family":"Lee","sequence":"additional","affiliation":[{"name":"Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB\u00a0T1J\u00a04B1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim","family":"McAllister","sequence":"additional","affiliation":[{"name":"Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB\u00a0T1J\u00a04B1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"rg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2004.02465.x"},{"key":"rg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb09068.x"},{"key":"rg3\/ref3","doi-asserted-by":"crossref","first-page":"51","DOI":"10.4141\/CJAS07040","volume":"88","author":"Baines D.","year":"2008","journal-title":"Can. 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