{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T17:57:32Z","timestamp":1784138252655,"version":"3.55.0"},"reference-count":61,"publisher":"Canadian Science Publishing","issue":"12","license":[{"start":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T00:00:00Z","timestamp":1228089600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:p>Although innate and adaptive immunity both play a role in amoebiasis, the mechanisms involved in the elimination of Entamoeba histolytica are poorly understood. To provide more information about the innate immune mechanisms that may confer protection against invasive amoebiasis, we administered inflammatory substances (bacillus Calmette-Gu\u00e9rin, lipopolysaccharide, complete Freund\u2019s adjuvant, or mineral oil) into the peritoneum of hamsters. The animals were then challenged with pathogenic trophozoites of E. histolytica and, after 7\u00a0days, the protective host response was analysed. We found that the nonspecific inflammatory response induced in the peritoneum was sufficient to prevent liver invasion by E. histolytica. In vitro experiments showed that the killing of trophozoites was mediated by peritoneal macrophages and a protein of 68\u00a0kDa with peroxidase activity.<\/jats:p>","DOI":"10.1139\/w08-106","type":"journal-article","created":{"date-parts":[[2008,12,18]],"date-time":"2008-12-18T16:56:17Z","timestamp":1229619377000},"page":"1032-1042","source":"Crossref","is-referenced-by-count":6,"title":["Innate immunity prevents tissue invasion by<i>Entamoeba histolytica<\/i>"],"prefix":"10.1139","volume":"54","author":[{"given":"Mineko","family":"Shibayama","sequence":"first","affiliation":[{"name":"Department of Experimental Pathology, CINVESTAV-IPN, Av. IPN No.\u00a02508 Zacatenco, CP\u00a007360, Mexico, DF."},{"name":"Department of Microbiology, UBIPRO, FES-Iztacala, UNAM, Avenida de los Barrios s\/n, Tlalnepantla Edo. de M\u00e9xico, CP 54090, Mexico, DF."},{"name":"Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis y Diaz Miron, CP\u00a011340, Mexico, DF."},{"name":"Department of Morphology, School of Medicine, IPN, Plan de San Luis y Diaz Miron, CP\u00a011340, Mexico, DF."},{"name":"Laboratory of Electron Microscopy, Mexican Faculty of Medicine, La Salle University, Fuentes 17, Tlalpan, CP\u00a014000, Mexico, DF."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00edctor","family":"Rivera-Aguilar","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology, CINVESTAV-IPN, Av. IPN No.\u00a02508 Zacatenco, CP\u00a007360, Mexico, DF."},{"name":"Department of Microbiology, UBIPRO, FES-Iztacala, UNAM, Avenida de los Barrios s\/n, Tlalnepantla Edo. de M\u00e9xico, CP 54090, Mexico, DF."},{"name":"Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis y Diaz Miron, CP\u00a011340, Mexico, DF."},{"name":"Department of Morphology, School of Medicine, IPN, Plan de San Luis y Diaz Miron, CP\u00a011340, Mexico, DF."},{"name":"Laboratory of Electron Microscopy, Mexican Faculty of Medicine, La Salle University, Fuentes 17, Tlalpan, CP\u00a014000, Mexico, DF."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Barbosa-Cabrera","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology, CINVESTAV-IPN, Av. IPN No.\u00a02508 Zacatenco, CP\u00a007360, Mexico, DF."},{"name":"Department of Microbiology, UBIPRO, FES-Iztacala, UNAM, Avenida de los Barrios s\/n, Tlalnepantla Edo. de M\u00e9xico, CP 54090, Mexico, DF."},{"name":"Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis y Diaz Miron, CP\u00a011340, Mexico, DF."},{"name":"Department of Morphology, School of Medicine, IPN, Plan de San Luis y Diaz Miron, CP\u00a011340, Mexico, DF."},{"name":"Laboratory of Electron Microscopy, Mexican Faculty of Medicine, La Salle University, Fuentes 17, Tlalpan, CP\u00a014000, Mexico, DF."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sa\u00fal","family":"Rojas-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology, CINVESTAV-IPN, Av. IPN No.\u00a02508 Zacatenco, CP\u00a007360, Mexico, DF."},{"name":"Department of Microbiology, UBIPRO, FES-Iztacala, UNAM, Avenida de los Barrios s\/n, Tlalnepantla Edo. de M\u00e9xico, CP 54090, Mexico, DF."},{"name":"Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis y Diaz Miron, CP\u00a011340, Mexico, DF."},{"name":"Department of Morphology, School of Medicine, IPN, Plan de San Luis y Diaz Miron, CP\u00a011340, Mexico, DF."},{"name":"Laboratory of Electron Microscopy, Mexican Faculty of Medicine, La Salle University, Fuentes 17, Tlalpan, CP\u00a014000, Mexico, DF."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Jarillo-Luna","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology, CINVESTAV-IPN, Av. IPN No.\u00a02508 Zacatenco, CP\u00a007360, Mexico, DF."},{"name":"Department of Microbiology, UBIPRO, FES-Iztacala, UNAM, Avenida de los Barrios s\/n, Tlalnepantla Edo. de M\u00e9xico, CP 54090, Mexico, DF."},{"name":"Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis y Diaz Miron, CP\u00a011340, Mexico, DF."},{"name":"Department of Morphology, School of Medicine, IPN, Plan de San Luis y Diaz Miron, CP\u00a011340, Mexico, DF."},{"name":"Laboratory of Electron Microscopy, Mexican Faculty of Medicine, La Salle University, Fuentes 17, Tlalpan, CP\u00a014000, Mexico, DF."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00edctor","family":"Tsutsumi","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology, CINVESTAV-IPN, Av. IPN No.\u00a02508 Zacatenco, CP\u00a007360, Mexico, DF."},{"name":"Department of Microbiology, UBIPRO, FES-Iztacala, UNAM, Avenida de los Barrios s\/n, Tlalnepantla Edo. de M\u00e9xico, CP 54090, Mexico, DF."},{"name":"Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis y Diaz Miron, CP\u00a011340, Mexico, DF."},{"name":"Department of Morphology, School of Medicine, IPN, Plan de San Luis y Diaz Miron, CP\u00a011340, Mexico, DF."},{"name":"Laboratory of Electron Microscopy, Mexican Faculty of Medicine, La Salle University, Fuentes 17, Tlalpan, CP\u00a014000, Mexico, DF."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Pacheco-Yepez","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology, CINVESTAV-IPN, Av. IPN No.\u00a02508 Zacatenco, CP\u00a007360, Mexico, DF."},{"name":"Department of Microbiology, UBIPRO, FES-Iztacala, UNAM, Avenida de los Barrios s\/n, Tlalnepantla Edo. de M\u00e9xico, CP 54090, Mexico, DF."},{"name":"Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis y Diaz Miron, CP\u00a011340, Mexico, DF."},{"name":"Department of Morphology, School of Medicine, IPN, Plan de San Luis y Diaz Miron, CP\u00a011340, Mexico, DF."},{"name":"Laboratory of Electron Microscopy, Mexican Faculty of Medicine, La Salle University, Fuentes 17, Tlalpan, CP\u00a014000, Mexico, DF."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Campos-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology, CINVESTAV-IPN, Av. IPN No.\u00a02508 Zacatenco, CP\u00a007360, Mexico, DF."},{"name":"Department of Microbiology, UBIPRO, FES-Iztacala, UNAM, Avenida de los Barrios s\/n, Tlalnepantla Edo. de M\u00e9xico, CP 54090, Mexico, DF."},{"name":"Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis y Diaz Miron, CP\u00a011340, Mexico, DF."},{"name":"Department of Morphology, School of Medicine, IPN, Plan de San Luis y Diaz Miron, CP\u00a011340, Mexico, DF."},{"name":"Laboratory of Electron Microscopy, Mexican Faculty of Medicine, La Salle University, Fuentes 17, Tlalpan, CP\u00a014000, Mexico, DF."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"rg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2006.06.014"},{"key":"rg2\/ref2","first-page":"97","volume":"72","author":"Anonymous","year":"1997","journal-title":"Wkly. Epidemiol. Rec."},{"key":"rg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.8.4522-4529.2005"},{"key":"rg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1991.tb00613.x"},{"key":"rg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1098"},{"key":"rg6\/ref6","doi-asserted-by":"crossref","first-page":"412","DOI":"10.4269\/ajtmh.1994.50.412","volume":"50","author":"Caballero-Salcedo A.","year":"1994","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"rg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2005.00743.x"},{"key":"rg8\/ref8","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1128\/IAI.12.1.36-41.1975","volume":"12","author":"Cole P.","year":"1975","journal-title":"Infect. Immun."},{"key":"rg9\/ref9","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1002\/jlb.46.3.270","volume":"46","author":"Denis M.","year":"1989","journal-title":"J. Leukoc. Biol."},{"key":"rg10\/ref10","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1128\/IAI.57.6.1750-1756.1989","volume":"57","author":"Denis M.","year":"1989","journal-title":"Infect. Immun."},{"key":"rg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(92)90053-Q"},{"key":"rg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(78)90144-X"},{"key":"rg13\/ref13","doi-asserted-by":"publisher","DOI":"10.1086\/381272"},{"key":"rg14\/ref14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1982.tb00433.x"},{"key":"rg15\/ref15","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S0171-2985(11)80498-5","volume":"188","author":"Ghadirian E.","year":"1993","journal-title":"Immunobiology"},{"key":"rg16\/ref16","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1128\/IAI.51.1.263-267.1986","volume":"51","author":"Ghadirian E.","year":"1986","journal-title":"Infect. Immun."},{"key":"rg17\/ref17","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1080\/00034983.1990.11812512","volume":"84","author":"Gold D.","year":"1990","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"rg18\/ref18","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(82)90118-X"},{"key":"rg19\/ref19","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1093\/infdis\/143.1.83","volume":"143","author":"Guerrant R.L.","year":"1981","journal-title":"J. Infect. Dis."},{"key":"rg20\/ref20","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.4049\/jimmunol.177.2.1208","volume":"177","author":"Hamano S.","year":"2006","journal-title":"J. Immunol."},{"key":"rg21\/ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2005.08.009"},{"key":"rg22\/ref22","doi-asserted-by":"publisher","DOI":"10.1086\/320740"},{"key":"rg23\/ref23","doi-asserted-by":"publisher","DOI":"10.1086\/341566"},{"key":"rg24\/ref24","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1002\/jlb.45.6.523","volume":"45","author":"Hirata K.","year":"1989","journal-title":"J. Leukoc. Biol."},{"key":"rg25\/ref25","doi-asserted-by":"crossref","first-page":"4496","DOI":"10.4049\/jimmunol.169.8.4496","volume":"169","author":"Houpt E.R.","year":"2002","journal-title":"J. Immunol."},{"key":"rg26\/ref26","doi-asserted-by":"publisher","DOI":"10.1007\/s100960050143"},{"key":"rg27\/ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2006.12.006"},{"key":"rg28\/ref28","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4894(02)00021-8"},{"key":"rg29\/ref29","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1080\/00034983.1966.11686397","volume":"60","author":"Jarumilinta R.","year":"1966","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"rg30\/ref30","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199203000-00007"},{"key":"rg31\/ref31","first-page":"709","volume":"47","author":"Katayama I.","year":"1982","journal-title":"Immunology"},{"key":"rg32\/ref32","doi-asserted-by":"publisher","DOI":"10.1038\/35095558"},{"key":"rg33\/ref33","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1204697"},{"key":"rg34\/ref34","doi-asserted-by":"crossref","first-page":"3999","DOI":"10.4049\/jimmunol.148.12.3999","volume":"148","author":"Lin J.Y.","year":"1992","journal-title":"J. Immunol."},{"key":"rg35\/ref35","doi-asserted-by":"crossref","first-page":"1534","DOI":"10.1128\/IAI.62.5.1534-1541.1994","volume":"62","author":"Lin J.Y.","year":"1994","journal-title":"Infect. Immun."},{"key":"rg36\/ref36","first-page":"203","volume":"59","author":"Matsuo S.","year":"1985","journal-title":"Clin. Exp. Immunol."},{"key":"rg37\/ref37","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1128\/IAI.42.1.362-367.1983","volume":"42","author":"McCuskey R.S.","year":"1983","journal-title":"Infect. Immun."},{"key":"rg38\/ref38","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(81)90038-4"},{"key":"rg39\/ref39","doi-asserted-by":"publisher","DOI":"10.1136\/ard.62.suppl_2.ii17"},{"key":"rg40\/ref40","unstructured":"Ortiz-Ortiz, L., Ruiz, B., and Gonzalez, A. 1990. Cellular immune responses.InAmebiasis: infection and disease byEntamoeba histolytica.Edited byR.R. Kretschmer. CRC Press, Boca Raton, Florida. pp.\u00a077\u2013103."},{"key":"rg41\/ref41","doi-asserted-by":"publisher","DOI":"10.1007\/s004360000287"},{"key":"rg42\/ref42","first-page":"259","volume":"17","author":"Perez-Tamayo R.","year":"1986","journal-title":"Arch. Invest. Med. (Mex.)"},{"key":"rg43\/ref43","doi-asserted-by":"publisher","DOI":"10.1086\/313493"},{"key":"rg44\/ref44","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-005-4503-z"},{"key":"rg45\/ref45","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1093\/infdis\/151.5.804","volume":"151","author":"Ravdin J.I.","year":"1985","journal-title":"J. Infect. Dis."},{"key":"rg46\/ref46","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/0002-9343(91)90555-C","volume":"90","author":"Reed S.L.","year":"1991","journal-title":"Am. J. Med."},{"key":"rg47\/ref47","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.3.1011"},{"key":"rg48\/ref48","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111998"},{"key":"rg49\/ref49","doi-asserted-by":"crossref","first-page":"3951","DOI":"10.1128\/IAI.65.9.3951-3953.1997","volume":"65","author":"Seydel K.B.","year":"1997","journal-title":"Infect. Immun."},{"key":"rg50\/ref50","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1128\/IAI.68.1.400-402.2000","volume":"68","author":"Seydel K.B.","year":"2000","journal-title":"Infect. Immun."},{"key":"rg51\/ref51","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1998.4218"},{"key":"rg52\/ref52","doi-asserted-by":"publisher","DOI":"10.1016\/S0188-4409(00)00187-9"},{"key":"rg53\/ref53","doi-asserted-by":"publisher","DOI":"10.1017\/S003118200600182X"},{"key":"rg54\/ref54","doi-asserted-by":"crossref","first-page":"9694","DOI":"10.1016\/S0021-9258(18)67570-2","volume":"261","author":"Thomas E.L.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"rg55\/ref55","doi-asserted-by":"publisher","DOI":"10.1016\/j.arcmed.2005.09.011"},{"key":"rg56\/ref56","first-page":"81","volume":"117","author":"Tsutsumi V.","year":"1984","journal-title":"Am. J. Pathol."},{"key":"rg57\/ref57","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.2.294-309.2002"},{"key":"rg58\/ref58","first-page":"230","volume":"28","author":"Velazquez C.","year":"1997","journal-title":"Arch. Med. Res."},{"key":"rg59\/ref59","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1128\/IAI.63.3.1089-1094.1995","volume":"63","author":"Wang W.","year":"1995","journal-title":"Infect. Immun."},{"key":"rg60\/ref60","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-05-2152"},{"key":"rg61\/ref61","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1996.9940"}],"container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/full-xml\/10.1139\/W08-106","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/W08-106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,23]],"date-time":"2023-05-23T01:54:41Z","timestamp":1684806881000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/W08-106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12]]},"references-count":61,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2008,12,1]]}},"alternative-id":["10.1139\/W08-106"],"URL":"https:\/\/doi.org\/10.1139\/w08-106","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,12]]}}}