{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T17:43:28Z","timestamp":1648662208173},"reference-count":29,"publisher":"Canadian Science Publishing","issue":"6","license":[{"start":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T00:00:00Z","timestamp":1243814400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:p>Leptospirosis is a widespread zoonotic disease caused by pathogenic leptospires. The identification of outer membrane proteins (OMPs) conserved among pathogenic leptospires, which are exposed on the leptospiral surface and expressed during mammalian infection, has become a major focus of leptospirosis research. pL40, a 40\u00a0kDa protein coded by the LA3744 gene in Leptospira interrogans , was found to be unique to Leptospira . Triton X-114 fractionation and flow cytometry analyses indicate that pL40 is a component of the leptospiral outer membrane. The conservation of pL40 among Leptospira strains prevalent in China was confirmed by both Western blotting and PCR screening. Furthermore, the pL40 antigen could be recognized by sera from guinea pigs and mice infected with low-passage L. interrogans. These findings indicate that pL40 may serve as a useful serodiagnostic antigen and vaccine candidate for L. interrogans.<\/jats:p>","DOI":"10.1139\/w09-014","type":"journal-article","created":{"date-parts":[[2009,9,18]],"date-time":"2009-09-18T15:59:37Z","timestamp":1253289577000},"page":"739-749","source":"Crossref","is-referenced-by-count":0,"title":["Molecular characterization of the pL40 protein in<i>Leptospira interrogans<\/i>"],"prefix":"10.1139","volume":"55","author":[{"given":"Wei","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai\u00a0200025, China."},{"name":"Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai\u00a0200032, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Yan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai\u00a0200025, China."},{"name":"Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai\u00a0200032, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang-Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai\u00a0200025, China."},{"name":"Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai\u00a0200032, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Qiang","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai\u00a0200025, China."},{"name":"Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai\u00a0200032, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bao-Yu","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai\u00a0200025, China."},{"name":"Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai\u00a0200032, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-Ping","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai\u00a0200025, China."},{"name":"Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai\u00a0200032, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-Hong","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai\u00a0200025, China."},{"name":"Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai\u00a0200032, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Kui","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai\u00a0200025, China."},{"name":"Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai\u00a0200032, China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"rg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.5.2414-2421.2003"},{"key":"rg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2003.10.004"},{"key":"rg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.8.4853-4863.2005"},{"key":"rg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2004.07.018"},{"key":"rg5\/ref5","unstructured":"Faine, S., Adler, B., Bolin, C., and Perolat, P. 1999.Leptospiraand leptospirosis. Medisci, Melbourne, Australia."},{"key":"rg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.9.4936-4945.2002"},{"key":"rg7\/ref7","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.1128\/IAI.66.4.1579-1587.1998","volume":"66","author":"Haake D.A.","year":"1998","journal-title":"Infect. Immun."},{"key":"rg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.4.2276-2285.2000"},{"key":"rg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01639-07"},{"key":"rg10\/ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2004.12.021"},{"key":"rg11\/ref11","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1128\/JB.94.1.27-31.1967","volume":"94","author":"Johnson R.C.","year":"1967","journal-title":"J. Bacteriol."},{"key":"rg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.29162-0"},{"key":"rg13\/ref13","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1016\/S0002-9440(10)63198-7","volume":"164","author":"Nally J.E.","year":"2004","journal-title":"Am. J. Pathol."},{"key":"rg14\/ref14","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.6.3251-3260.2005"},{"key":"rg15\/ref15","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.10.7014-7017.2005"},{"key":"rg16\/ref16","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2004000400003"},{"key":"rg17\/ref17","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e32814a5729"},{"key":"rg18\/ref18","doi-asserted-by":"publisher","DOI":"10.1038\/nature01597"},{"key":"rg19\/ref19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030097"},{"key":"rg20\/ref20","first-page":"53","volume":"25","author":"Sakolvaree Y.","year":"2007","journal-title":"Asian Pac. J. Allergy Immunol."},{"key":"rg21\/ref21","doi-asserted-by":"crossref","first-page":"3174","DOI":"10.1128\/IAI.63.8.3174-3181.1995","volume":"63","author":"Shang E.S.","year":"1995","journal-title":"Infect. Immun."},{"key":"rg22\/ref22","doi-asserted-by":"crossref","first-page":"2322","DOI":"10.1128\/IAI.64.6.2322-2330.1996","volume":"64","author":"Shang E.S.","year":"1996","journal-title":"Infect. Immun."},{"key":"rg23\/ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2007.10.012"},{"key":"rg24\/ref24","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001188"},{"key":"rg25\/ref25","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-181"},{"key":"rg26\/ref26","unstructured":"World Health Organization. 2003. Human leptospirosis: guidance for diagnosis, surveillance and control. V."},{"key":"rg27\/ref27","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-7-293"},{"key":"rg28\/ref28","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7270.2005.00094.x"},{"key":"rg29\/ref29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7254.2005.00075.x"}],"container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/full-xml\/10.1139\/W09-014","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/W09-014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,22]],"date-time":"2020-05-22T09:33:34Z","timestamp":1590140014000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/W09-014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6]]},"references-count":29,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2009,6]]}},"alternative-id":["10.1139\/W09-014"],"URL":"https:\/\/doi.org\/10.1139\/w09-014","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,6]]}}}