{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T22:55:56Z","timestamp":1777416956697,"version":"3.51.4"},"reference-count":38,"publisher":"Canadian Science Publishing","issue":"1","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:p>Escherichia coli can be used to help identify sources of fecal contamination in the environment. Escherichia coli genotypic fecal libraries and pattern-matching algorithms were assessed for their effectiveness in correctly identifying sources. Fecal samples (n = 172) were collected from various sources from three agricultural landscapes in Canada. Escherichia coli isolates were fingerprinted using BOX- and enterobacterial repetitive intergenic consensus (ERIC) - polymerase chain reaction primers, revealing 769 and 1 057 distinct genotypes, respectively, for the 9 047 isolates collected in 2004 in Ontario. The average rate of correct classification (ARCC) was comparable for BOX- (48%) and ERIC-based (62%) libraries and between libraries with clones removed per sample (55%) and clones removed per unit (54%). ARCC increased with fewer classification units (from 44% to 65%). ARCC for k-nearest neighbour (64%) and maximum similarity (60%) algorithms were comparable, but maximum similarity had better sensitivity and specificity than k-nearest neighbour. Geographical and temporal shifts in community composition resulted in loss of accuracy. Several ERIC genotypes (n = 112) were common between sources and were removed from the library, improving ARCC (77%). The latter library proved to be more accurate, but its accuracy with respect to sourcing environmental isolates remains to be tested.<\/jats:p>","DOI":"10.1139\/w09-113","type":"journal-article","created":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T16:54:31Z","timestamp":1265043271000},"page":"8-17","source":"Crossref","is-referenced-by-count":15,"title":["Optimization and validation of rep-PCR genotypic libraries for microbial source tracking of environmental Escherichia coli isolates"],"prefix":"10.1139","volume":"56","author":[{"given":"Emilie","family":"Lyautey","sequence":"first","affiliation":[{"name":"Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V\u00a04T3, Canada."},{"name":"Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON K1A\u00a00C6, Canada."},{"name":"Environment Canada, 867 Lakeshore Road, Burlington, ON L7R\u00a04A6, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zexun","family":"Lu","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V\u00a04T3, Canada."},{"name":"Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON K1A\u00a00C6, Canada."},{"name":"Environment Canada, 867 Lakeshore Road, Burlington, ON L7R\u00a04A6, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David R.","family":"Lapen","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V\u00a04T3, Canada."},{"name":"Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON K1A\u00a00C6, Canada."},{"name":"Environment Canada, 867 Lakeshore Road, Burlington, ON L7R\u00a04A6, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanya E.","family":"Berkers","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V\u00a04T3, Canada."},{"name":"Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON K1A\u00a00C6, Canada."},{"name":"Environment Canada, 867 Lakeshore Road, Burlington, ON L7R\u00a04A6, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas A.","family":"Edge","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V\u00a04T3, Canada."},{"name":"Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON K1A\u00a00C6, Canada."},{"name":"Environment Canada, 867 Lakeshore Road, Burlington, ON L7R\u00a04A6, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward","family":"Topp","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V\u00a04T3, Canada."},{"name":"Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON K1A\u00a00C6, Canada."},{"name":"Environment Canada, 867 Lakeshore Road, Burlington, ON L7R\u00a04A6, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"rg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1021\/es034211q"},{"key":"rg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.12.5656-5667.2001"},{"issue":"3","key":"rg3\/ref3","first-page":"313","volume":"35","author":"Buchan A.","year":"2001","journal-title":"FEMS Microbiol. 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