{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:32:19Z","timestamp":1773275539275,"version":"3.50.1"},"reference-count":24,"publisher":"Canadian Science Publishing","issue":"10","license":[{"start":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T00:00:00Z","timestamp":1285891200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:p>The iron-oxidizing system of a moderately thermophilic, extremely acidophilic, gram-positive mixotroph, Sulfobacillus sibiricus N1<jats:sup>T<\/jats:sup>, was studied by spectroscopic, high-performance liquid chromatography and inhibitory analyses. Hemes B, A, and O were detected in membranes of S. sibiricus N1<jats:sup>T<\/jats:sup>. It is proposed that the electron transport chain from Fe<jats:sup>2+<\/jats:sup>to O<jats:sub>2<\/jats:sub>is terminated by 2 physiological oxidases: aa<jats:sub>3<\/jats:sub>-type cytochrome, which dominates in the early-exponential phase of growth, and bo<jats:sub>3<\/jats:sub>-type cytochrome, whose role in iron oxidation becomes more prominent upon growth of the culture. Both oxidases were sensitive to cyanide and azide. Cytochrome aa<jats:sub>3<\/jats:sub>was more sensitive to cyanide and azide, with K<jats:sub>i<\/jats:sub>values of 4.1\u00a0and 2.5\u00a0\u00b5mol\u00b7L<jats:sup>\u20131<\/jats:sup>, respectively, compared with K<jats:sub>i<\/jats:sub>values for cytochrome bo<jats:sub>3<\/jats:sub>, which were 9.5\u00a0\u00b5mol\u00b7L<jats:sup>\u20131<\/jats:sup>for cyanide and 7.0\u00a0\u00b5mol\u00b7L<jats:sup>\u20131<\/jats:sup>for azide. This is the first evidence for the participation of a bo<jats:sub>3<\/jats:sub>-type oxidase in ferrous iron oxidation. The respiratory chain of the mixotroph contains, in addition to the 2 terminal oxidases, a membrane-bound cytochrome b<jats:sub>573<\/jats:sub>.<\/jats:p>","DOI":"10.1139\/w10-063","type":"journal-article","created":{"date-parts":[[2010,10,20]],"date-time":"2010-10-20T18:38:32Z","timestamp":1287599912000},"page":"803-808","source":"Crossref","is-referenced-by-count":12,"title":["Ferrous iron oxidation in moderately thermophilic acidophile<i>Sulfobacillus sibiricus<\/i>N1<sup>T<\/sup>"],"prefix":"10.1139","volume":"56","author":[{"given":"Tatiana Y.","family":"Dinarieva","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Biology, Moscow State University, 1\/12 Lenin\u2019s Hills, Moscow 119992, Russian Federation."},{"name":"Winogradsky Institute of Microbiology, Russian Academy of Sciences, 7\/2 pr. 60-letiya Oktyabrya, Moscow 117312, Russian Federation."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna E.","family":"Zhuravleva","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Biology, Moscow State University, 1\/12 Lenin\u2019s Hills, Moscow 119992, Russian Federation."},{"name":"Winogradsky Institute of Microbiology, Russian Academy of Sciences, 7\/2 pr. 60-letiya Oktyabrya, Moscow 117312, Russian Federation."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oksana A.","family":"Pavlenko","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Biology, Moscow State University, 1\/12 Lenin\u2019s Hills, Moscow 119992, Russian Federation."},{"name":"Winogradsky Institute of Microbiology, Russian Academy of Sciences, 7\/2 pr. 60-letiya Oktyabrya, Moscow 117312, Russian Federation."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iraida A.","family":"Tsaplina","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Biology, Moscow State University, 1\/12 Lenin\u2019s Hills, Moscow 119992, Russian Federation."},{"name":"Winogradsky Institute of Microbiology, Russian Academy of Sciences, 7\/2 pr. 60-letiya Oktyabrya, Moscow 117312, Russian Federation."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander I.","family":"Netrusov","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Biology, Moscow State University, 1\/12 Lenin\u2019s Hills, Moscow 119992, Russian Federation."},{"name":"Winogradsky Institute of Microbiology, Russian Academy of Sciences, 7\/2 pr. 60-letiya Oktyabrya, Moscow 117312, Russian Federation."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"rg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01545-08"},{"key":"rg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02019-07"},{"key":"rg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1990.tb03781.x"},{"key":"rg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02589-06"},{"key":"rg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1993.tb00261.x"},{"key":"rg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M802496200"},{"key":"rg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28362-0"},{"key":"rg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1023\/A:1023742002493"},{"key":"rg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2008.17"},{"key":"rg10\/ref10","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27515-0"},{"issue":"1","key":"rg11\/ref11","first-page":"13","volume":"67","author":"Kondrat\u2019eva T.F.","year":"1998","journal-title":"Microbiology"},{"issue":"1","key":"rg12\/ref12","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","volume":"193","author":"Lowry O.H.","year":"1951","journal-title":"J. Biol. Chem."},{"issue":"34","key":"rg13\/ref13","doi-asserted-by":"crossref","first-page":"21473","DOI":"10.1016\/S0021-9258(17)31828-8","volume":"269","author":"L\u00fcbben M.","year":"1994","journal-title":"J. Biol. Chem."},{"key":"rg14\/ref14","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026007620113"},{"key":"atypb1\/ref15","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-394"},{"issue":"3","key":"rg16\/ref16","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1099\/00221287-146-3-551","volume":"146","author":"Richardson D.J.","year":"2000","journal-title":"Microbiology"},{"key":"rg17\/ref17","doi-asserted-by":"crossref","unstructured":"Rieske, J.S. 1967. The quantitative determination of mitochondrial hemoproteins. Methods Enzymol.10:\u00a0488\u2013493.","DOI":"10.1016\/0076-6879(67)10081-5"},{"key":"rg18\/ref18","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02799-07"},{"issue":"4","key":"rg19\/ref19","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1093\/oxfordjournals.jbchem.a022640","volume":"127","author":"Sone N.","year":"2000","journal-title":"J. Biochem."},{"key":"rg20\/ref20","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02795-8"},{"key":"rg21\/ref21","doi-asserted-by":"publisher","DOI":"10.1023\/B:MICI.0000023976.80874.ef"},{"key":"rg22\/ref22","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2001.02037.x"},{"key":"atypb2\/ref23","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-597"},{"key":"rg24\/ref24","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026039132408"}],"container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/full-xml\/10.1139\/W10-063","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/W10-063","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,12]],"date-time":"2021-11-12T06:18:47Z","timestamp":1636697927000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/W10-063"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10]]},"references-count":24,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2010,10]]}},"alternative-id":["10.1139\/W10-063"],"URL":"https:\/\/doi.org\/10.1139\/w10-063","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10]]}}}