{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:54:51Z","timestamp":1776387291517,"version":"3.51.2"},"reference-count":48,"publisher":"Canadian Science Publishing","issue":"12","license":[{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:p>Trophozoites of Entamoeba histolytica HM-1:IMSS become less virulent after long-term maintenance in axenic cultures. The factors responsible for the loss of virulence during in vitro cultivation remain unclear. However, it is known that in vitro cultivation of amoeba in culture medium supplemented with cholesterol restores their virulence. In this study, we analyzed the effect of adding phosphatidylcholine\u2013cholesterol (PC\u2013Chol) liposomes to the culture medium and evaluated the effect of this lipid on various biochemical and biological functions of E.\u00a0histolytica HM-1:IMSS in terms of its virulence. The addition of PC\u2013Chol liposomes to the culture medium maintained the virulence of these parasites against hamster liver at the same level as the original virulent E. histolytica strain, even though these amoebae were maintained without passage through hamster liver for 18\u00a0months. The trophozoites also showed increased endocytosis, erythrophagocytosis, and carbohydrate residue expression on the amoebic surface. Protease activities were also modified by the presence of cholesterol in the culture medium. These findings indicate the capacity of cholesterol to preserve amoeba virulence and provide an alternative method for the maintenance of virulent E.\u00a0histolytica trophozoites without the need for in vivo procedures.<\/jats:p>","DOI":"10.1139\/w10-088","type":"journal-article","created":{"date-parts":[[2010,12,22]],"date-time":"2010-12-22T14:41:15Z","timestamp":1293028875000},"page":"987-995","source":"Crossref","is-referenced-by-count":18,"title":["Effect of phosphatidylcholine\u2013cholesterol liposomes on Entamoeba histolytica virulence"],"prefix":"10.1139","volume":"56","author":[{"given":"Jes\u00fas","family":"Serrano-Luna","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Center for Research and Advanced Studies, Av. 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