{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T22:04:37Z","timestamp":1762898677689},"reference-count":35,"publisher":"Canadian Science Publishing","issue":"4","license":[{"start":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:00:00Z","timestamp":1301616000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:p>Yersinia pestis is a bacterium that is transmitted between fleas, which have a body temperature of 26\u00a0\u00b0C, and mammalian hosts, which have a body temperature of 37\u00a0\u00b0C. To adapt to the temperature shift, phenotype variations, including virulence, occur. In this study, an antigen microarray including 218 proteins of Y. pestis was used to evaluate antibody responses in a pooled plague serum that was unadsorbed, adsorbed by Y. pestis cultivated at 26\u00a0\u00b0C, or adsorbed by Y. pestis cultivated at 26\u00a0and\u00a037\u00a0\u00b0C to identify protein expression changes during the temperature shift. We identified 12 proteins as being expressed at 37\u00a0\u00b0C but not at 26\u00a0\u00b0C, or expressed at significantly higher levels at 37\u00a0\u00b0C than at 26\u00a0\u00b0C. The antibodies against 7 proteins in the serum adsorbed by Y. pestis cultivated at 26\u00a0and\u00a037\u00a0\u00b0C remained positive, suggesting that they were not expressed on the surface of Y. pestis in LB broth in vitro or specifically expressed in vivo. This study proved that protein microarray and antibody profiling comprise a promising technique for monitoring gene expression at the protein level and for better understanding pathogenicity, to find new vaccine targets against plague.<\/jats:p>","DOI":"10.1139\/w11-007","type":"journal-article","created":{"date-parts":[[2011,4,7]],"date-time":"2011-04-07T14:37:24Z","timestamp":1302187044000},"page":"287-294","source":"Crossref","is-referenced-by-count":8,"title":["Use of protein microarray to identify gene expression changes of<i>Yersinia pestis<\/i>at different temperatures"],"prefix":"10.1139","volume":"57","author":[{"given":"Bei","family":"Li","sequence":"first","affiliation":[{"name":"Taihe Hospital, Hubei Medical University, Shiyan, Hubei Province 442000, China."},{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yafang","family":"Tan","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyu","family":"Guo","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baizhong","family":"Cui","sequence":"additional","affiliation":[{"name":"Qinghai Institute for Endemic Diseases Prevention and Control, Xining, Qinghai Province 811602, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zuyun","family":"Wang","sequence":"additional","affiliation":[{"name":"Qinghai Institute for Endemic Diseases Prevention and Control, Xining, Qinghai Province 811602, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hu","family":"Wang","sequence":"additional","affiliation":[{"name":"Qinghai Institute for Endemic Diseases Prevention and Control, Xining, Qinghai Province 811602, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaobiao","family":"Guo","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziwen","family":"Zhu","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zongmin","family":"Du","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruifu","family":"Yang","sequence":"additional","affiliation":[{"name":"Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"issue":"11","key":"refg1\/ref1","doi-asserted-by":"crossref","first-page":"4580","DOI":"10.1128\/IAI.64.11.4580-4585.1996","volume":"64","author":"Anderson G.W.","year":"1996","journal-title":"Infect. 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