{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T20:36:12Z","timestamp":1790886972793,"version":"4.1.0"},"reference-count":25,"publisher":"Canadian Science Publishing","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:p>The low frequency of nontuberculous mycobacterial infections, nonspecific symptoms for individual mycobacteria, and the lack of specific identification methods could alter correct diagnosis. This study presents a combined microbiology and molecular-based approach for Mycobacterium\u00a0marinum detection in four aquarists with cutaneous mycobacterial infection. Simultaneously, ecology screening for M.\u00a0marinum presence in the aquarists\u2019 fish tanks was performed. A total of 38 mycobacterial isolates originated from four human patients (n\u00a0=\u00a020), aquarium animals (n\u00a0=\u00a08), and an aquarium environment (n\u00a0= 10). Isolate identification was carried out using 16S\u00a0rRNA sequence analysis. A microbiology-based approach, followed by 16S rRNA sequence analysis, was successfully used for detection of M.\u00a0marinum in all four patients. Animal and environmental samples were simultaneously examined, and a total of seven mycobacterial species were isolated: Mycobacterium chelonae , Mycobacterium fortuitum , Mycobacterium gordonae , Mycobacterium\u00a0kansasii , Mycobacterium mantenii , Mycobacterium marinum , and Mycobacterium\u00a0peregrinum . The presence of M.\u00a0marinum was proven in the aquarium environments of two patients. Although M.\u00a0marinum is described as being present in water, it was detected only in fish.<\/jats:p>","DOI":"10.1139\/w11-104","type":"journal-article","created":{"date-parts":[[2012,1,10]],"date-time":"2012-01-10T23:12:38Z","timestamp":1326237158000},"page":"39-44","source":"Crossref","is-referenced-by-count":23,"title":["<i>Mycobacterium marinum<\/i>infections in humans and tracing of its possible environmental sources"],"prefix":"10.1139","volume":"58","author":[{"given":"Michal","family":"Slany","sequence":"first","affiliation":[{"name":"Veterinary Research Institute, O.I.E. Reference laboratories for Paratuberculosis and Avian Tuberculosis, Brno, Czech Republic."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Jezek","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology and Parasitology, County Hospital Pribram, Czech Republic."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Fiserova","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology and Parasitology, County Hospital Pribram, Czech Republic."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Bodnarova","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, Charles University in Prague, First Faculty of Medicine and General University Hospital, Czech Republic."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiri","family":"Stork","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, Charles University in Prague, First Faculty of Medicine and General University Hospital, Czech Republic."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Havelkova","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Mycobacteria, National Institute of Public Health, Prague, Czech Republic."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frantisek","family":"Kalat","sequence":"additional","affiliation":[{"name":"Department of Surgery, County Hospital Pribram, Czech Republic."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivo","family":"Pavlik","sequence":"additional","affiliation":[{"name":"Veterinary Research Institute, O.I.E. Reference laboratories for Paratuberculosis and Avian Tuberculosis, Brno, Czech Republic."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/39.4.315"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.162.15.1746"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0560.2000.027003124.x"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1054\/bjps.1999.3245"},{"key":"refg5\/ref5","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/1471-2180-8-48","volume":"8","author":"Chimara E.","year":"2008","journal-title":"BMC Microbiol."},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-009-9401-0"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2009.04161.x"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1007\/BF02817414"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2008.05.012"},{"key":"refg10\/ref10","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/1471-2334-3-26","volume":"3","author":"Harmsen D.","year":"2003","journal-title":"BMC Infect. Dis."},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1097\/01.PRS.0000153197.64808.B9"},{"issue":"4","key":"refg12\/ref12","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1016\/S0749-0712(21)00422-4","volume":"14","author":"Hoyen H.A.","year":"1998","journal-title":"Hand Clin."},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.1086\/313972"},{"key":"refg14\/ref14","doi-asserted-by":"crossref","unstructured":"Kazda, J., Pavlik, I., Falkinham, J.O., III, and Hruska, K. (Editors). 2009. The ecology of mycobacteria: impact on animal\u2019s and human\u2019s health. 2nd end. Springer, Heidelberg.","DOI":"10.1007\/978-1-4020-9413-2"},{"key":"refg15\/ref15","doi-asserted-by":"publisher","DOI":"10.1086\/376628"},{"key":"refg16\/ref16","doi-asserted-by":"publisher","DOI":"10.1159\/000328220"},{"key":"refg17\/ref17","doi-asserted-by":"crossref","first-page":"343","DOI":"10.17221\/5789-VETMED","volume":"48","author":"Matlova L.","year":"2003","journal-title":"Vet. Med. Czech"},{"key":"refg18\/ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2007.10.006"},{"key":"refg19\/ref19","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2007.02467.x"},{"key":"refg20\/ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2006.11.014"},{"key":"refg21\/ref21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2009.01030.x"},{"key":"refg22\/ref22","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.017277-0"},{"key":"refg23\/ref23","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1997.18081891.x"},{"key":"refg24\/ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2004.10.003"},{"issue":"5","key":"refg25\/ref25","doi-asserted-by":"crossref","first-page":"337","DOI":"10.7883\/yoken.JJID.2006.337","volume":"59","author":"Tsai H.C.","year":"2006","journal-title":"Jpn. J. Infect. Dis."}],"container-title":["Canadian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/full-xml\/10.1139\/w11-104","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/w11-104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,17]],"date-time":"2024-04-17T14:21:10Z","timestamp":1713363670000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/w11-104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["10.1139\/w11-104"],"URL":"https:\/\/doi.org\/10.1139\/w11-104","relation":{},"ISSN":["0008-4166","1480-3275"],"issn-type":[{"value":"0008-4166","type":"print"},{"value":"1480-3275","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1]]}}}