{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:11:00Z","timestamp":1791292260715,"version":"4.1.0"},"reference-count":10,"publisher":"Canadian Science Publishing","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:p> New Delhi metallo-\u03b2-lactamase-1 (NDM-1) is a novel type of metallo-\u03b2-lactamase (MBL) responsible for bacterial resistance to \u03b2-lactam antibiotics. Acinetobacter junii was previously shown to possess a MBL phenotype; however, the genes responsible for this phenotype were not identified. In this study, we reported the identification of NDM-1 gene in a clinical isolate of A. junii from a child patient in China, which was resistant to all \u03b2-lactams except aztreonam but sensitive to aminoglycosides and quinolones. The cloned NDM-1 gene contained an open reading frame of 813 bp and had a nucleotide sequence 99.9% identical (812\/813) to reported NDM-1 genes carried by Acinetobacter baumannii , Enterococcus faecium , Escherichia coli , and Klebsiella pneumoniae . Recombinant NDM-1 protein was successfully expressed in E.\u00a0coli BL21, and antibiotic sensitivities of the NDM-1-producing E.\u00a0coli were largely similar to the A.\u00a0junii 1454 isolate. The findings of this study raise attention to the emergence and spread of NDM-1-carrying bacteria in China. <\/jats:p>","DOI":"10.1139\/w11-112","type":"journal-article","created":{"date-parts":[[2012,1,10]],"date-time":"2012-01-10T18:12:38Z","timestamp":1326219158000},"page":"112-115","source":"Crossref","is-referenced-by-count":28,"title":["Identification of New Delhi metallo-\u03b2-lactamase gene (NDM-1) from a clinical isolate of <i>Acinetobacter junii<\/i> in China"],"prefix":"10.1139","volume":"58","author":[{"given":"Zhenwen","family":"Zhou","sequence":"first","affiliation":[{"name":"Guangzhou Women and Children\u2019s Medical Center, Guangzhou Medical College, 318 Renminzhong Road, Guangzhou 510120, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruili","family":"Guan","sequence":"additional","affiliation":[{"name":"Guangzhou Women and Children\u2019s Medical Center, Guangzhou Medical College, 318 Renminzhong Road, Guangzhou 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