{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T18:02:18Z","timestamp":1772992938016,"version":"3.50.1"},"reference-count":37,"publisher":"Canadian Science Publishing","issue":"9","license":[{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:p> Vaccination is the most promising strategy to reduce the incidence of pneumococcal infection. Although there are vaccines available, all of them are based on polysaccharide antigens (conjugated or not). In addition to their high cost, those vaccines do not cover all serotypes. To overcome these hindrances, we evaluated the immunogenicity and the protective efficacy of the S9 ribosomal protein of Streptococcus pneumoniae with the aim of developing a protein-based vaccine in the future. The gene encoding the S9 ribosomal protein was cloned in pET21-a expression vector, and the recombinant S9 protein was used to immunize mice. Significantly higher levels of anti-S9 immunoglobulin G were achieved (with predominance of immunoglobulin G1) in comparison with the control. Antibodies elicited against S.\u00a0pneumoniae protein extract in rabbit recognized the recombinant S9 protein by Western blot, thus demonstrating its immunogenicity. Moreover, mice immunized with recombinant S9 protein and challenged with a virulent strain of S.\u00a0pneumoniae presented a significant reduction of bacteremia after 24\u00a0h of infection as compared with the control. However, in the S9-immunized mice the onset of death was insignificantly delayed, but all of them died by the fourth day postinfection. <\/jats:p>","DOI":"10.1139\/w2012-083","type":"journal-article","created":{"date-parts":[[2012,8,28]],"date-time":"2012-08-28T01:55:24Z","timestamp":1346118924000},"page":"1055-1062","source":"Crossref","is-referenced-by-count":6,"title":["Immune response and protective efficacy of S9 ribosomal protein of <i>Streptococcus pneumoniae<\/i> in a model of sepsis"],"prefix":"10.1139","volume":"58","author":[{"given":"Helton Fernandes","family":"Ara\u00fajo","sequence":"first","affiliation":[{"name":"Research and Development Center, Ezequiel Dias Foundation (Funed), Belo Horizonte, MG, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patr\u00edcia Cota","family":"Campos","sequence":"additional","affiliation":[{"name":"Research and Development Center, Ezequiel Dias Foundation (Funed), Belo Horizonte, MG, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dhian Renato Almeida","family":"Camargo","sequence":"additional","affiliation":[{"name":"Otavio Magalh\u00e3es Institute Ezequiel Dias Foundation (Funed), Belo Horizonte, MG, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filippe Nonato Roberto","family":"Pereira","sequence":"additional","affiliation":[{"name":"Research and Development Center, Ezequiel Dias Foundation (Funed), Belo Horizonte, MG, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle Lara","family":"Samuel","sequence":"additional","affiliation":[{"name":"Otavio Magalh\u00e3es Institute Ezequiel Dias Foundation (Funed), Belo Horizonte, MG, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marluce Aparecida Assun\u00e7\u00e3o","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Otavio Magalh\u00e3es Institute Ezequiel Dias Foundation (Funed), Belo Horizonte, MG, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Consuelo Latorre","family":"Fortes-Dias","sequence":"additional","affiliation":[{"name":"Research and Development Center, Ezequiel Dias Foundation (Funed), Belo Horizonte, MG, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie Yvette","family":"Leclercq","sequence":"additional","affiliation":[{"name":"Research and Development Center, Ezequiel Dias Foundation (Funed), Belo Horizonte, MG, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-5-58"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.04.069"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(02)02351-X"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025558"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.12.126"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01199-09"},{"key":"refg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2006.03047.x"},{"key":"refg8\/ref8","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00400-07"},{"key":"refg9\/ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2010.02.009"},{"key":"refg10\/ref10","doi-asserted-by":"publisher","DOI":"10.1021\/pr800196v"},{"key":"refg11\/ref11","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01516-08"},{"key":"refg12\/ref12","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071168"},{"key":"refg13\/ref13","doi-asserted-by":"publisher","DOI":"10.1086\/505117"},{"key":"refg14\/ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2008.06.002"},{"key":"refg15\/ref15","doi-asserted-by":"publisher","DOI":"10.1089\/aid.2007.0009"},{"key":"refg16\/ref16","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(03)00167-7"},{"key":"refg17\/ref17","doi-asserted-by":"publisher","DOI":"10.1093\/bfgp\/4.3.241"},{"key":"refg18\/ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2005.06.004"},{"key":"refg19\/ref19","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2133653100"},{"key":"refg20\/ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.01.066"},{"key":"refg21\/ref21","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(11)70090-1"},{"key":"refg22\/ref22","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01878.x"},{"key":"refg23\/ref23","doi-asserted-by":"publisher","DOI":"10.1086\/521028"},{"key":"refg24\/ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2011.08.013"},{"key":"refg25\/ref25","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.23.14503"},{"key":"refg27\/ref27","first-page":"493","volume":"27","author":"Reed L.J.","year":"1938","journal-title":"Am. 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