{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T14:57:08Z","timestamp":1751468228782},"reference-count":44,"publisher":"Canadian Science Publishing","issue":"4","license":[{"start":{"date-parts":[[2000,4,1]],"date-time":"2000-04-01T00:00:00Z","timestamp":954547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Microbiol."],"published-print":{"date-parts":[[2000,4,1]]},"abstract":"<jats:p>The properties of both Ca<jats:sup>2+<\/jats:sup>influx and efflux in the mycelium during the life cycle of Trichoderma viride were studied by means of<jats:sup>45<\/jats:sup>Ca<jats:sup>2+<\/jats:sup>and by X-ray fluorescence spectroscopy measurements. The properties of the<jats:sup>45<\/jats:sup>Ca<jats:sup>2+<\/jats:sup>influx and effluxes indicate that they are mediated by different transport systems. The Ca<jats:sup>2+<\/jats:sup>influx could be mediated by an electrogenic Ca<jats:sup>2+<\/jats:sup>\/nH<jats:sup>+<\/jats:sup>antiport, or by an Ca<jats:sup>2+<\/jats:sup>uniport system. Both Ca<jats:sup>2+<\/jats:sup>influx and efflux were stimulated by the uncouplers (and the treatment leading to the suppression of energy metabolism) and by azalomycin F, an antifungal agent. Salicylate stimulated the Ca<jats:sup>2+<\/jats:sup>efflux, but inhibited the Ca<jats:sup>2+<\/jats:sup>influx. In the isolated preparation of crude vacuolar\/mitochondrial fraction, salicylate induced the Ca<jats:sup>2+<\/jats:sup>release, as did A23187. Azalomycin F moderately released Ca<jats:sup>2+<\/jats:sup>from the microsomal fraction. On the other hand, uncouplers did not release Ca<jats:sup>2+<\/jats:sup>from the isolated organelles, but inhibited to a different extent the ATP-dependent and -independent Ca<jats:sup>2+<\/jats:sup>influx. The results could be explained in terms of the capacitative Ca<jats:sup>2+<\/jats:sup>influx mechanism. The rate of<jats:sup>45<\/jats:sup>Ca<jats:sup>2+<\/jats:sup>influx, or of the<jats:sup>40<\/jats:sup>Ca<jats:sup>2+<\/jats:sup>content, was maximal after about 30 h of submerged cultivation, and then decreased. The results show that loading of internal Ca<jats:sup>2+<\/jats:sup>stores occurs in the early stages of the development of mycelium only, and the Ca<jats:sup>2+<\/jats:sup>influx mechanism is developmentally down-regulated, being almost nonexistent during its later stages. In older mycelium, growth seems to be autonomous of the extracellular Ca<jats:sup>2+<\/jats:sup>until the onset of conidiation.Key words: Trichoderma viride, development, Ca<jats:sup>2+<\/jats:sup>influx, Ca<jats:sup>2+<\/jats:sup>efflux, salicylate, uncoupler, azalomycin F.<\/jats:p>","DOI":"10.1139\/w99-139","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T06:10:03Z","timestamp":1303625403000},"page":"312-324","source":"Crossref","is-referenced-by-count":1,"title":["Ca<sup>2+<\/sup>fluxes in developing<i>Trichoderma viride<\/i>mycelium"],"prefix":"10.1139","volume":"46","author":[{"given":"Martin","family":"\u008a\u008aimkovi\u010d","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svetlana","family":"Kry\u009a\u009atofov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L'udov\u00edt","family":"Vare\u010dka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"p_1\/p_1_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/00362178485380031","volume":"22","author":"Alsina A.","year":"1984","journal-title":"Sabouraudia"},{"key":"p_2\/p_2_1","first-page":"2341","volume":"133","author":"Cornelius G.","year":"1987","journal-title":"J. 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