{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:40:54Z","timestamp":1792096854614,"version":"4.4.0"},"reference-count":25,"publisher":"Canadian Science Publishing","issue":"12","license":[{"start":{"date-parts":[[2003,12,1]],"date-time":"2003-12-01T00:00:00Z","timestamp":1070236800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2003,12,1]]},"abstract":"<jats:p>An investigation was made of the radiosensitivity of the toxic and immunological properties of Androctonus australis hector venom. This venom was irradiated with two doses of gamma rays (1 and 2 kGy) from a<jats:sup>60<\/jats:sup>Co source. The results showed that venom toxicity was abolished for the two radiation doses (1 and 2 kGy) with, respectively, 10 and 25 times its initial LD50 value. However, irradiated venoms were immunogenic, and the antibodies elicited by them were able to recognize the native venom by enzyme-linked immunosorbent assay. Antisera raised against these toxoids (1 and 2 kGy) had a higher neutralizing capacity and immunoreactivity against all components of native venom than did the antiserum produced against the native venom. The antiserum of rabbits immunized with 2-kGy-irradiated venom was more efficient than 1-kGy-irradiated toxoid antiserum. Indeed, in vivo protection assays showed that the mice immunized with 2-kGy-irradiated venom resisted lethal doses (i.p.) of A. australis hector venom.Key words: venom, Androctonus australis hector, gamma irradiation, immunogenicity, in vivo protection.<\/jats:p>","DOI":"10.1139\/y03-115","type":"journal-article","created":{"date-parts":[[2004,1,19]],"date-time":"2004-01-19T16:41:11Z","timestamp":1074530471000},"page":"1118-1124","source":"Crossref","is-referenced-by-count":21,"title":["Effect of gamma irradiation on toxicity and immunogenicity of<i>Androctonus australis hector<\/i>venom"],"prefix":"10.1139","volume":"81","author":[{"given":"Lila","family":"Abib","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima","family":"Laraba-Djebari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"p_2\/p_2_1","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1007\/BF02023142","volume":"177","author":"Behrens B.","year":"1935","journal-title":"Arch. 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