{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:05:02Z","timestamp":1761487502522},"reference-count":40,"publisher":"Canadian Science Publishing","issue":"8-9","license":[{"start":{"date-parts":[[2005,8,1]],"date-time":"2005-08-01T00:00:00Z","timestamp":1122854400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2005,8,1]]},"abstract":"<jats:p>Chloride (Cl<jats:sup>\u2013<\/jats:sup>) efflux induces depolarization and contraction of vascular smooth muscle cells. In the basilar arteries from the New Zealand white rabbits, the role of Cl<jats:sup>\u2013<\/jats:sup>flux in serotonin-induced contraction was demonstrated by (i) inhibition of Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2013<\/jats:sup>co-transporter (NKCC1) to decreased Cl<jats:sup>\u2013<\/jats:sup>influx with bumetanide; (ii) a disabled Cl<jats:sup>\u2013<\/jats:sup>\/HCO<jats:sub>3<\/jats:sub>\u2013 exchanger with bicarbonate free HEPES solution; (iii) blockade of Cl<jats:sup>\u2013<\/jats:sup>channels using 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) and indanyloxyacetic acid 94, R-(+)-methylindazone (R-(+)-IAA-94); and (iv) substitution of extracellular Cl<jats:sup>\u2013<\/jats:sup>with methanesulfonate acid (113 mmol\/L; Cl<jats:sup>\u2013<\/jats:sup>, 10 mmol\/L). In addition, the expression of NKCC1 in brain tissues after neonatal hypoxia-ischemia was examined at mRNA and protein levels using RT-PCR and Western blotting techniques. NKCC1 mRNA and protein expressions were increased at 24 and 48 h and returned to normal levels at 72 h after hypoxia insult when compared with the control littermates. In conclusion, Cl<jats:sup>\u2013<\/jats:sup>efflux regulates cerebral circulation and the up-regulation of NKCC1 after neonatal hypoxia-ischemia may contribute to brain injury. Key words: serotonin, NCCK1, neonatal hypoxia.<\/jats:p>","DOI":"10.1139\/y05-076","type":"journal-article","created":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T20:35:46Z","timestamp":1133901346000},"page":"767-773","source":"Crossref","is-referenced-by-count":21,"title":["Role of Cl<sup>\u2013<\/sup>in cerebral vascular tone and expression of Na<sup>+<\/sup>-K<sup>+<\/sup>-2Cl<sup>\u2013<\/sup>co-transporter after neonatal hypoxia-ischemia"],"prefix":"10.1139","volume":"83","author":[{"given":"Yun","family":"Dai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiping","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John H","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"p_1\/p_1_1","doi-asserted-by":"crossref","first-page":"C1383","DOI":"10.1152\/ajpcell.1999.276.6.C1383","volume":"276","author":"Akar F.","year":"1999","journal-title":"Am. 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