{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:52:47Z","timestamp":1791294767885,"version":"4.1.0"},"reference-count":35,"publisher":"Canadian Science Publishing","issue":"11","license":[{"start":{"date-parts":[[2005,11,1]],"date-time":"2005-11-01T00:00:00Z","timestamp":1130803200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2005,11,1]]},"abstract":"<jats:p>Unlike T cells, the role of natural killer (NK) cells is not well documented in the concanavalin (ConA)- induced hepatitis model. This study aimed to investigate the regulatory effect of high levels of interferon-\u03b3 (IFN-\u03b3) on NK cells in ConA-induced hepatitis. The cytotoxicities of NK cells from ConA-injected mice or NK cell lines (NK92 and NKL) were detected by the 4-h<jats:sup>51<\/jats:sup>Cr release assay. Depletion of NK cells with AsGM1 antibody was used to assess the NK cell role in ConA-induced hepatitis. Expression of NK cell receptors and cytotoxic molecules was measured by reverse transcription \u2013 polymerase chain reaction. Twelve hours after ConA injection, serum IFN-\u03b3 was significantly increased in wild mice, but not in severe combined immunodeficiency mice, and hepatic NK cells exerted impaired cytotoxicity against YAC-l cells in wild mice. Eight hours after NK cells were incubated in serum from ConA-treated mice, NK cell cytotoxicity was down-modulated and the effect was abolished by pretreatment with neutralizing serum IFN-\u03b3 with specific antibody in vitro. A high concentration of IFN-\u03b3 (&gt; 1000 U\/mL) inhibited the cytotoxicities of 2 NK cell lines in vitro, accompanied with down-regulation of NKG2D transcripts and up-regulation of NKG2A\/B and KIR2DL transcripts. The inhibitive role of IFN-\u03b3 was not seen in NKG2D ligand negative cells. These results suggest that NK cell cytotoxicity was inhibited by high levels of IFN-\u03b3 in ConA-induced hepatitis, which may relate to the dispensable role of NK cells.Key words: cytotoxicity, hepatoimmunology, interferon-\u03b3, liver injury.<\/jats:p>","DOI":"10.1139\/y05-093","type":"journal-article","created":{"date-parts":[[2006,1,6]],"date-time":"2006-01-06T15:23:11Z","timestamp":1136560991000},"page":"1045-1053","source":"Crossref","is-referenced-by-count":12,"title":["Impaired NK cell cytotoxicity by high level of interferon-\u03b3 in concanavalin A-induced hepatitis"],"prefix":"10.1139","volume":"83","author":[{"given":"Zhongjun","family":"Dong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cai","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiming","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigang","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"p_1\/p_1_1","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/S0168-8278(02)00022-3","volume":"36","author":"Abe T.","year":"2002","journal-title":"J. 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