{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T12:58:46Z","timestamp":1782997126533,"version":"3.54.5"},"reference-count":24,"publisher":"Canadian Science Publishing","issue":"5","license":[{"start":{"date-parts":[[2006,5,1]],"date-time":"2006-05-01T00:00:00Z","timestamp":1146441600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:p>Cold ischemia \u2013 warm reperfusion (CI\/WR) injury of liver transplantation involves hepatocyte cell death, the nature and underlying mechanisms of which remain unclear. Isolated hepatocytes and isolated perfused livers were used to determine the prevalence of necrosis and apoptosis as well as mitochondrial dysfunction. In isolated cells, propidium iodide and Hoechst 33342 staining showed a cold-storage, time-dependent increase in necrosis, whereas apoptosis was minimal even after 48\u00a0h of hypothermia. Nonetheless, a progressive loss of mitochondrial membrane potential was observed. Translocation of mitochondrial cytochrome c toward microsomes occurred within 24\u00a0h of CI\/WR, with cytochrome c reaching the cytosol later. Mitochondria isolated from whole livers subjected to CI\/WR also display reduced metabolic parameters and increased susceptibility to swelling. These events are associated with increased activity of major initiator (caspase 9) and effector (caspase 3) caspases. The results demonstrate that CI\/WR induces mitochondrial dysfunction in isolated cells and in the whole organ; only in the latter is that sufficient to trigger the classical mitochondrial pathway of apoptosis. Our study also provides evidence for the involvement of endoplasmic reticulum stress in CI\/WR hepatocyte injury. Combined protection of mitochondria and endoplasmic reticulum may thus represent an innovative therapeutic avenue to enhance liver graft viability and functional integrity.<\/jats:p>","DOI":"10.1139\/y06-014","type":"journal-article","created":{"date-parts":[[2010,10,22]],"date-time":"2010-10-22T18:34:47Z","timestamp":1287772487000},"page":"547-554","source":"Crossref","is-referenced-by-count":30,"title":["Implication of mitochondrial dysfunction and cell death in cold preservation \u2013 warm reperfusion-induced hepatocyte injury"],"prefix":"10.1139","volume":"84","author":[{"given":"Marjolaine","family":"Duval","sequence":"first","affiliation":[{"name":"D\u00e9partement de Pharmacologie et Groupe d\u2019\u00e9tude sur les prot\u00e9ines membranaires, Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Montr\u00e9al, P.O. Box 6128, Downtown Station, Montr\u00e9al, QC H3C 3J7, Canada."},{"name":"INSERM U660 et Universit\u00e9 Paris XII, Cr\u00e9teil, F-94010, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Plin","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Pharmacologie et Groupe d\u2019\u00e9tude sur les prot\u00e9ines membranaires, Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Montr\u00e9al, P.O. Box 6128, Downtown Station, Montr\u00e9al, QC H3C 3J7, Canada."},{"name":"INSERM U660 et Universit\u00e9 Paris XII, Cr\u00e9teil, F-94010, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aziz","family":"Elimadi","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Pharmacologie et Groupe d\u2019\u00e9tude sur les prot\u00e9ines membranaires, Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Montr\u00e9al, P.O. Box 6128, Downtown Station, Montr\u00e9al, QC H3C 3J7, Canada."},{"name":"INSERM U660 et Universit\u00e9 Paris XII, Cr\u00e9teil, F-94010, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane","family":"Vallerand","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Pharmacologie et Groupe d\u2019\u00e9tude sur les prot\u00e9ines membranaires, Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Montr\u00e9al, P.O. Box 6128, Downtown Station, Montr\u00e9al, QC H3C 3J7, Canada."},{"name":"INSERM U660 et Universit\u00e9 Paris XII, Cr\u00e9teil, F-94010, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Paul","family":"Tillement","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Pharmacologie et Groupe d\u2019\u00e9tude sur les prot\u00e9ines membranaires, Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Montr\u00e9al, P.O. Box 6128, Downtown Station, Montr\u00e9al, QC H3C 3J7, Canada."},{"name":"INSERM U660 et Universit\u00e9 Paris XII, Cr\u00e9teil, F-94010, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Morin","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Pharmacologie et Groupe d\u2019\u00e9tude sur les prot\u00e9ines membranaires, Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Montr\u00e9al, P.O. Box 6128, Downtown Station, Montr\u00e9al, QC H3C 3J7, Canada."},{"name":"INSERM U660 et Universit\u00e9 Paris XII, Cr\u00e9teil, F-94010, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre S.","family":"Haddad","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Pharmacologie et Groupe d\u2019\u00e9tude sur les prot\u00e9ines membranaires, Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Montr\u00e9al, P.O. 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