{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T21:56:47Z","timestamp":1762898207344},"reference-count":48,"publisher":"Canadian Science Publishing","issue":"6","license":[{"start":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T00:00:00Z","timestamp":1149120000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:p>Magnesium (Mg) deficiency and oxidative stress are independently implicated in the etiopathogenesis of various cardiovascular disorders. This study was undertaken to examine the hypothesis that Mg deficiency augments the myocardial response to oxidative stress. Electrically stimulated rat papillary muscle was used for recording the contractile variation. Biochemical variables of energy metabolism (adenosine triphosphate (ATP) and creatine phosphate) and markers of tissue injury (lactate dehydrogenase (LDH) release and lipidperoxidation), which can affect myocardial contractility, were assayed in Langendorff-perfused rat hearts. Hydrogen peroxide (100\u00a0\u00b5mol\/L) was used as the source of reactive oxygen species. The negative inotropic response to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>was significantly higher in Mg deficiency (0.48\u00a0mmol Mg\/L) than in Mg sufficiency (1.2\u00a0mmol Mg\/L). Low Mg levels did not affect ATP levels or tissue lipid peroxidation. However, H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>induced a decrease in ATP; enhanced lipid peroxidation and the release of LDH were augmented by Mg deficiency. Increased lipid peroxidation associated with a decrease in available energy might be responsible for the augmentation of the negative inotropic response to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>in Mg deficiency. The observations from this study validate the hypothesis that myocardial response to oxidative stress is augmented by Mg deficiency. This observation has significance in ischemia\u2013reperfusion injury, where Mg deficiency can have an additive effect on the debilitating consequences.<\/jats:p>","DOI":"10.1139\/y06-017","type":"journal-article","created":{"date-parts":[[2006,8,9]],"date-time":"2006-08-09T16:11:13Z","timestamp":1155139873000},"page":"617-624","source":"Crossref","is-referenced-by-count":8,"title":["Magnesium deficiency augments myocardial response to reactive oxygen species"],"prefix":"10.1139","volume":"84","author":[{"given":"L.","family":"Manju","sequence":"first","affiliation":[{"name":"Division of Cellular and Molecular Cardiology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695 011, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. Renuka","family":"Nair","sequence":"additional","affiliation":[{"name":"Division of Cellular and Molecular Cardiology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695 011, India."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"atypb1\/ref1","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1038\/1931256a0","volume":"193","author":"Arrigoni O.","year":"1962","journal-title":"Nature (London)"},{"key":"atypb2\/ref2","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1996.0265"},{"key":"atypb3\/ref3","first-page":"7","volume":"16","author":"Busserolles J.","year":"2003","journal-title":"Magnes. Res."},{"key":"atypb4\/ref4","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1079\/BJN2001498","volume":"87","author":"Bussiere F.I.","year":"2002","journal-title":"Br. J. Nutr."},{"key":"atypb5\/ref5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1989.tb15063.x"},{"key":"atypb6\/ref6","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(62)90008-6"},{"key":"atypb7\/ref7","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2828(92)91049-B"},{"key":"atypb8\/ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2004.01.034"},{"key":"atypb9\/ref9","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(96)00089-7"},{"key":"atypb10\/ref10","doi-asserted-by":"crossref","first-page":"1800","DOI":"10.1172\/JCI114085","volume":"83","author":"Goldhaber J.I.","year":"1989","journal-title":"J. Clin. Invest."},{"key":"atypb11\/ref11","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1093\/ajhp\/61.15.1569","volume":"61","author":"Gums J.G.","year":"2004","journal-title":"Am. J. Health Syst. Pharm."},{"key":"atypb12\/ref12","first-page":"213","volume":"3","author":"Gunther T.","year":"1989","journal-title":"J. Trace Elem. Electrolytes Health Dis."},{"key":"atypb13\/ref13","first-page":"1275","volume":"40","author":"Hans C.P.","year":"2002","journal-title":"Indian J. Exp. Biol."},{"key":"atypb14\/ref14","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1152\/ajpheart.1998.275.3.H917","volume":"275","author":"Headrick J.P.","year":"1998","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"atypb15\/ref15","first-page":"2784","volume":"45","author":"Hecht S.M.","year":"1986","journal-title":"Fed. Proc."},{"key":"atypb16\/ref16","unstructured":"Heinz, F., and Weiber, H. 1985. Metabolites: lipids, amino acids and related compounds.InMethods of enzymatic analysis. 3rd ed.Edited byH.U. Bergmeyer. Weinheim and Academic Press, New York. pp.\u00a0507\u2013514."},{"key":"atypb17\/ref17","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1161\/01.CIR.100.7.690","volume":"100","author":"Ide T.","year":"1999","journal-title":"Circulation"},{"key":"atypb18\/ref18","first-page":"H1225","volume":"251","author":"Jackson C.V.","year":"1986","journal-title":"Am. J. Physiol."},{"key":"atypb19\/ref19","doi-asserted-by":"crossref","first-page":"C179","DOI":"10.1152\/ajpcell.1994.266.1.C179","volume":"266","author":"Janero D.R.","year":"1994","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"atypb20\/ref20","doi-asserted-by":"crossref","first-page":"2354","DOI":"10.1016\/S0021-9258(18)52251-1","volume":"266","author":"Josephson R.A.","year":"1991","journal-title":"J. Biol. Chem."},{"key":"atypb21\/ref21","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1093\/ajcn\/75.3.550","volume":"75","author":"Klevay L.M.","year":"2002","journal-title":"Am. J. Clin. Nutr."},{"key":"atypb22\/ref22","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1177\/153537020322800604","volume":"228","author":"Kramer J.H.","year":"2003","journal-title":"Exp. Biol. Med."},{"key":"atypb23\/ref23","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/BF02783317","volume":"60","author":"Kumar B.P.","year":"1997","journal-title":"Biol. Trace Elem. Res."},{"key":"atypb24\/ref24","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(96)00124-0"},{"key":"atypb25\/ref25","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706206"},{"key":"atypb26\/ref26","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","volume":"193","author":"Lowry O.H.","year":"1951","journal-title":"J. Biol. Chem."},{"key":"atypb27\/ref27","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1007\/BF02535212","volume":"24","author":"Mahfouz M.M.","year":"1989","journal-title":"Lipids"},{"key":"atypb28\/ref28","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.2002.2050"},{"key":"atypb29\/ref29","doi-asserted-by":"publisher","DOI":"10.1006\/phrs.1997.0177"},{"key":"atypb30\/ref30","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2828(88)90578-0"},{"key":"atypb31\/ref31","first-page":"118","volume":"8","author":"Nair R.R.","year":"1995","journal-title":"Natl. Med. J. India"},{"key":"atypb32\/ref32","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1161\/01.RES.73.4.758","volume":"73","author":"Nakamura T.Y.","year":"1993","journal-title":"Circ. Res."},{"key":"atypb33\/ref33","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1968.tb00428.x"},{"key":"atypb34\/ref34","unstructured":"Poole-Wilson, P.A. 1983. The mechanism of cell death in heart muscle after hypoxia or ischemia.InMyocardial ischemia and protection.Edited byH. Refsun, P. Jynge, and O.D. Mjos. Churchill Livingstone, New York. pp. 123\u2013130."},{"key":"atypb35\/ref35","unstructured":"Seelig, M.S. 1980 Magnesium deficiency in the pathogenesis of disease \u2014 early roots of cardiovascular, skeletal and renal abnormalities. Plenum Medical Book Co. New York."},{"key":"atypb36\/ref36","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1080\/07315724.1994.10718432","volume":"13","author":"Seelig M.S.","year":"1994","journal-title":"J. Am. Coll. Nutr."},{"key":"atypb37\/ref37","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2828(74)90024-8"},{"key":"atypb38\/ref38","doi-asserted-by":"publisher","DOI":"10.1096\/fj.00-0176com"},{"key":"atypb39\/ref39","doi-asserted-by":"crossref","first-page":"131","DOI":"10.3109\/10715769209049166","volume":"16","author":"Suzuki Y.J.","year":"1992","journal-title":"Free Radic. Res. Commun."},{"key":"atypb40\/ref40","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1994.1055"},{"key":"atypb41\/ref41","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(03)00285-2"},{"key":"atypb42\/ref42","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1080\/15287399409531859","volume":"41","author":"Toraason M.","year":"1994","journal-title":"J. Toxicol. Environ. Health"},{"key":"atypb43\/ref43","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(96)00278-X"},{"key":"atypb44\/ref44","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(02)01065-1"},{"key":"atypb45\/ref45","doi-asserted-by":"publisher","DOI":"10.1007\/s003950050055"},{"key":"atypb46\/ref46","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1093\/cvr\/26.9.851","volume":"26","author":"Yanagi S.","year":"1992","journal-title":"Cardiovasc. Res."},{"key":"atypb47\/ref47","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1093\/cvr\/20.8.597","volume":"20","author":"Ytrehus K.","year":"1986","journal-title":"Cardiovasc. Res."},{"key":"atypb48\/ref48","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/0005-2728(92)90022-T","volume":"1140","author":"Yuan G.","year":"1992","journal-title":"Biochim. Biophys. Acta"}],"container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/full-xml\/10.1139\/y06-017","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y06-017","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,31]],"date-time":"2021-07-31T08:37:17Z","timestamp":1627720637000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y06-017"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,6]]},"references-count":48,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,6]]}},"alternative-id":["10.1139\/y06-017"],"URL":"https:\/\/doi.org\/10.1139\/y06-017","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,6]]}}}