{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T18:53:21Z","timestamp":1793040801298,"version":"4.5.0"},"reference-count":25,"publisher":"Canadian Science Publishing","issue":"3-4","license":[{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:p>Inflammation plays an important role in vessel wall remodeling that occurs in atherosclerosis and postangioplasty restenosis. Monocytic chemoattractant protein-1 (MCP-1) is one of the main attractors of monocytes and some lymphocyte subsets to the damaged vessel. The aims of the study were to confirm MCP-1 participation in the development of acute coronary syndromes, to produce the potential MCP-1 peptide antagonist, and to investigate its effects in vitro and in vivo in different animal models of inflammation. MCP-1 plasma concentration was measured by ELISA (enzyme-linked immunosorbent assay). Chemokine receptor expression by cells isolated from human atherosclerotic lesions was assessed by direct immunofluorescence and flow cytometry. MCP-1 sequence was analyzed with Peptide Companion software and peptides were synthesized using Fmoc strategy. The peptide resistance to degradation was checked by<jats:sup>1<\/jats:sup>H-NMR spectroscopy. The peptide effect on MCP-1-stimulated cell migration was studied in Boyden chamber and in mouse air pouch model, and its influence on lipopolysaccharide (LPS)-induced inflammatory cell recruitment was investigated in models of subcutaneous inflammation in rats and nonhuman primates. We revealed nearly a 2-fold increase of MCP-1 plasma level in patients with unstable angina in comparison with patients with stable angina. The atherosclerotic plaque specimens obtained from patients with unstable angina contained a significant amount of chemokine receptor-expressing leukocytes. Peptide from MCP-1 C-terminal 65\u201376 sequence (peptide X) inhibited MCP-1-stimulated monocytic cell migration in vitro and in vivo. Peptide X labeled with<jats:sup>99m<\/jats:sup>Tc accumulated specifically at sites of inflammation in rats. Peptide X administrated i.m and i.v. suppressed monocyte and granulocyte recruitment induced by subcutaneous injection of LPS in the back of rats and non-human primates. Our data demonstrate that MCP-1-mediated chemotaxis could be responsible for atherosclerotic plaque \u201cdestabilization\u201d. Peptide X may represent a new class of anti-inflammatory drugs to be used in cardiology.<\/jats:p>","DOI":"10.1139\/y07-008","type":"journal-article","created":{"date-parts":[[2007,7,6]],"date-time":"2007-07-06T00:30:03Z","timestamp":1183681803000},"page":"332-340","source":"Crossref","is-referenced-by-count":7,"title":["The peptide analogue of MCP-1 65\u201376 sequence is an inhibitor of inflammationThis paper is one of a selection of papers published in this Special Issue, entitled The Cellular and Molecular Basis of Cardiovascular Dysfunction, Dhalla 70th Birthday Tribute."],"prefix":"10.1139","volume":"85","author":[{"given":"Evgeny I.","family":"Chazov","sequence":"first","affiliation":[{"name":"Russian Cardiology Research Complex, 3 Cherepkovskaya, 15a, Moscow 121552 Russia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janna D.","family":"Bespalova","sequence":"additional","affiliation":[{"name":"Russian Cardiology Research Complex, 3 Cherepkovskaya, 15a, Moscow 121552 Russia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana I.","family":"Arefieva","sequence":"additional","affiliation":[{"name":"Russian Cardiology Research Complex, 3 Cherepkovskaya, 15a, Moscow 121552 Russia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadezhda B.","family":"Kukhtina","sequence":"additional","affiliation":[{"name":"Russian Cardiology Research Complex, 3 Cherepkovskaya, 15a, Moscow 121552 Russia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria V.","family":"Sidorova","sequence":"additional","affiliation":[{"name":"Russian Cardiology Research Complex, 3 Cherepkovskaya, 15a, Moscow 121552 Russia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey I.","family":"Provatorov","sequence":"additional","affiliation":[{"name":"Russian Cardiology Research Complex, 3 Cherepkovskaya, 15a, Moscow 121552 Russia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana L.","family":"Krasnikova","sequence":"additional","affiliation":[{"name":"Russian Cardiology Research Complex, 3 Cherepkovskaya, 15a, Moscow 121552 Russia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"155","reference":[{"key":"atypb1\/ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(84)90232-1"},{"key":"atypb2\/ref2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(20000201)39:2<158::AID-CYTO9>3.0.CO;2-8"},{"key":"atypb3\/ref3","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000146672.10582.17"},{"key":"atypb4\/ref4","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(98)00318-9"},{"key":"atypb5\/ref5","doi-asserted-by":"publisher","DOI":"10.1007\/BF01081522"},{"key":"atypb6\/ref6","first-page":"1227","volume":"142","author":"Dieter Geratz J.","year":"1993","journal-title":"Am. J. Pathol."},{"key":"atypb7\/ref7","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80139-2"},{"key":"atypb8\/ref8","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1016\/S0022-2275(20)33509-4","volume":"40","author":"Han K.H.","year":"1999","journal-title":"J. Lipid Res."},{"key":"atypb9\/ref9","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(01)00636-0"},{"key":"atypb10\/ref10","doi-asserted-by":"publisher","DOI":"10.1007\/s10630-005-0149-z"},{"key":"atypb11\/ref11","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2796.2000.00654.x"},{"key":"atypb12\/ref12","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.537878"},{"key":"atypb13\/ref13","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199802123380706"},{"key":"atypb14\/ref14","doi-asserted-by":"publisher","DOI":"10.1136\/heart.85.5.571"},{"key":"atypb15\/ref15","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0334864100"},{"key":"atypb16\/ref16","doi-asserted-by":"publisher","DOI":"10.1042\/0264-6021:3400803"},{"key":"atypb17\/ref17","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2001.01228.x"},{"key":"atypb18\/ref18","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1182\/blood.V90.3.909","volume":"90","author":"Rollins B.J.","year":"1997","journal-title":"Blood"},{"key":"atypb19\/ref19","first-page":"582","volume":"30","author":"Sidorova M.B.","year":"2004","journal-title":"Bioorg. Khim."},{"key":"atypb20\/ref20","first-page":"161","volume":"32","author":"Sidorova M.V.","year":"2006","journal-title":"Bioorg. Khim."},{"key":"atypb21\/ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.ehj.2004.06.011"},{"key":"atypb22\/ref22","doi-asserted-by":"crossref","first-page":"1838","DOI":"10.1096\/fj.02-0094fje","volume":"16","author":"Usui M.","year":"2002","journal-title":"FASEB J."},{"key":"atypb23\/ref23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1993.tb17920.x"},{"key":"atypb24\/ref24","doi-asserted-by":"crossref","first-page":"S10","DOI":"10.5551\/jat1994.1.Supplemment1_S10","volume":"1","author":"Wilcox J.N.","year":"1994","journal-title":"J. Atheroscler. Thromb."},{"key":"atypb25\/ref25","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00088-9"}],"container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/full-xml\/10.1139\/Y07-008","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/Y07-008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,16]],"date-time":"2021-08-16T17:43:00Z","timestamp":1629135780000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/Y07-008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3]]},"references-count":25,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[2007,3]]}},"alternative-id":["10.1139\/Y07-008"],"URL":"https:\/\/doi.org\/10.1139\/y07-008","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,3]]}}}