{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T13:10:32Z","timestamp":1788354632283,"version":"build-2803163510"},"reference-count":26,"publisher":"Canadian Science Publishing","issue":"2","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:p>Recent investigations involving intact rabbit renal proximal tubules indicated that organic anion transporter 3 (OAT3) may be involved in the transport of 2,3-dimercapto-1-propanesulfonic acid (DMPS). Therefore, we evaluated the interaction of OAT3 with DMPS to determine the effect of OAT3 on basolateral DMPS uptake. We used stably transfected HEK293 cells expressing human and rabbit orthologs of the exchanger OAT1 and OAT3. Using 6-carboxyfluorescein (6-CF) as a substrate, the IC<jats:sub>50<\/jats:sub>determinations for reduced DMPS (DMPSH) revealed a stronger interaction with OAT1 than with OAT3 (rbOAT1, 123.3\u00a0\u00b1 13.7; hOAT1, 85.1\u00a0\u00b1 8.8; rbOAT3, 171.7\u00a0\u00b1 22.3; and hOAT3, 172.2\u00a0\u00b1 36.4\u00a0\u00b5mol\/L). However, inhibition of 6-CF uptake by the oxidized form of DMPS (DMPSS), the main form of DMPS in the blood, showed a greater affinity for OAT3 (rbOAT1, 237.4\u00a0\u00b1 23; hOAT1, 104.6\u00a0\u00b1 13.1; rbOAT3, 52.4\u00a0\u00b1 7.6; and hOAT3, 31.6\u00a0\u00b1 6.6\u00a0\u00b5mol\/L). To determine whether DMPSH and DMPSS are substrates for OAT3, we performed efflux studies with [<jats:sup>14<\/jats:sup>C]glutarate and inwardly directed gradients of glutarate. The inhibitors trans-stimulated the efflux of [<jats:sup>14<\/jats:sup>C]glutarate, suggesting that OAT3 may be able to transport both forms of DMPS. On the basis of the substantial interaction of OAT3 with DMPSS, we conclude that OAT3 represents the dominant basolateral player in renal detoxification processes resulting from use of DMPS.<\/jats:p>","DOI":"10.1139\/y09-123","type":"journal-article","created":{"date-parts":[[2010,3,17]],"date-time":"2010-03-17T13:48:33Z","timestamp":1268833713000},"page":"141-146","source":"Crossref","is-referenced-by-count":14,"title":["Organic anion transporter 3 (OAT3) and renal transport of the metal chelator 2,3-dimercapto-1-propanesulfonic acid (DMPS)"],"prefix":"10.1139","volume":"88","author":[{"given":"Matthias","family":"R\u00f6diger","sequence":"first","affiliation":[{"name":"Zentrum f\u00fcr Physiologie und Pathophysiologie, Abt. Vegetative Physiologie, Humboldtallee 23, Universit\u00e4t G\u00f6ttingen, G\u00f6ttingen 37073, Germany."},{"name":"Zentrum Zahn-, Mund- und Kieferheilkunde, Abteilung Prothetik, Universit\u00e4tsklinikum G\u00f6ttingen, G\u00f6ttingen 37073, Germany."},{"name":"Department of Physiology, College of Medicine, University of Arizona, Tucson, AZ 85724, USA."},{"name":"Klinikum der Universit\u00e4t M\u00fcnchen, I. Frauenklinik-Innenstadt, Maistr. 11, M\u00fcnchen 80337, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Zentrum f\u00fcr Physiologie und Pathophysiologie, Abt. Vegetative Physiologie, Humboldtallee 23, Universit\u00e4t G\u00f6ttingen, G\u00f6ttingen 37073, Germany."},{"name":"Zentrum Zahn-, Mund- und Kieferheilkunde, Abteilung Prothetik, Universit\u00e4tsklinikum G\u00f6ttingen, G\u00f6ttingen 37073, Germany."},{"name":"Department of Physiology, College of Medicine, University of Arizona, Tucson, AZ 85724, USA."},{"name":"Klinikum der Universit\u00e4t M\u00fcnchen, I. Frauenklinik-Innenstadt, Maistr. 11, M\u00fcnchen 80337, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Ugele","sequence":"additional","affiliation":[{"name":"Zentrum f\u00fcr Physiologie und Pathophysiologie, Abt. Vegetative Physiologie, Humboldtallee 23, Universit\u00e4t G\u00f6ttingen, G\u00f6ttingen 37073, Germany."},{"name":"Zentrum Zahn-, Mund- und Kieferheilkunde, Abteilung Prothetik, Universit\u00e4tsklinikum G\u00f6ttingen, G\u00f6ttingen 37073, Germany."},{"name":"Department of Physiology, College of Medicine, University of Arizona, Tucson, AZ 85724, USA."},{"name":"Klinikum der Universit\u00e4t M\u00fcnchen, I. Frauenklinik-Innenstadt, Maistr. 11, M\u00fcnchen 80337, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaus","family":"Gersdorff","sequence":"additional","affiliation":[{"name":"Zentrum f\u00fcr Physiologie und Pathophysiologie, Abt. Vegetative Physiologie, Humboldtallee 23, Universit\u00e4t G\u00f6ttingen, G\u00f6ttingen 37073, Germany."},{"name":"Zentrum Zahn-, Mund- und Kieferheilkunde, Abteilung Prothetik, Universit\u00e4tsklinikum G\u00f6ttingen, G\u00f6ttingen 37073, Germany."},{"name":"Department of Physiology, College of Medicine, University of Arizona, Tucson, AZ 85724, USA."},{"name":"Klinikum der Universit\u00e4t M\u00fcnchen, I. Frauenklinik-Innenstadt, Maistr. 11, M\u00fcnchen 80337, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen H.","family":"Wright","sequence":"additional","affiliation":[{"name":"Zentrum f\u00fcr Physiologie und Pathophysiologie, Abt. Vegetative Physiologie, Humboldtallee 23, Universit\u00e4t G\u00f6ttingen, G\u00f6ttingen 37073, Germany."},{"name":"Zentrum Zahn-, Mund- und Kieferheilkunde, Abteilung Prothetik, Universit\u00e4tsklinikum G\u00f6ttingen, G\u00f6ttingen 37073, Germany."},{"name":"Department of Physiology, College of Medicine, University of Arizona, Tucson, AZ 85724, USA."},{"name":"Klinikum der Universit\u00e4t M\u00fcnchen, I. Frauenklinik-Innenstadt, Maistr. 11, M\u00fcnchen 80337, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Burckhardt","sequence":"additional","affiliation":[{"name":"Zentrum f\u00fcr Physiologie und Pathophysiologie, Abt. 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