{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T11:03:05Z","timestamp":1761217385706},"reference-count":33,"publisher":"Canadian Science Publishing","issue":"8","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:p> Using immunofluorescence and real 3-D confocal microscopy, our results showed the presence of ET-1, ET<jats:sub>A<\/jats:sub>, and ET<jats:sub>B<\/jats:sub> receptors in isolated human aortic vascular endothelial cells (hVECs). The level of the peptide and its receptors was significantly higher in the nucleus (including the nuclear envelope membranes) than in the cytosol (including the cell membrane). Furthermore, using the Western blot technique we demonstrated the presence of both ET<jats:sub>A<\/jats:sub> and ET<jats:sub>B<\/jats:sub> receptors. Using intact and isolated human hVECs and the Fura-2 calcium (Ca<jats:sup>2+<\/jats:sup>) measurement technique, we showed that ET-1 induced a dose-dependent increase of total intracellular free Ca<jats:sup>2+<\/jats:sup>, with an EC<jats:sub>50<\/jats:sub> of 1.3\u00a0\u00d7 10<jats:sup>\u221210<\/jats:sup>\u00a0mol\/L. The specific ET<jats:sub>A<\/jats:sub> receptor antagonist ABT-627 (10<jats:sup>\u22127<\/jats:sup>\u00a0mol\/L), but not the ET<jats:sub>B<\/jats:sub> receptor antagonist A-192621 (10<jats:sup>\u22127<\/jats:sup>\u00a0mol\/L), prevented the ET-1 (10<jats:sup>\u22129<\/jats:sup>\u00a0mol\/L) induced increase of total intracellular Ca<jats:sup>2+<\/jats:sup>. In conclusion, these results clearly show that similar to ET<jats:sub>B<\/jats:sub> receptors, ET<jats:sub>A<\/jats:sub> receptors are also present in human aortic vascular endothelial cells and their levels are higher than ET<jats:sub>B<\/jats:sub> in the nucleus when compared with the cytosol. Furthermore, we suggest that ET<jats:sub>A<\/jats:sub>, but not ET<jats:sub>B<\/jats:sub>, receptors mediate the effect of ET-1 on total intracellular Ca<jats:sup>2+<\/jats:sup> of human aortic vascular endothelial cells. <\/jats:p>","DOI":"10.1139\/y10-057","type":"journal-article","created":{"date-parts":[[2010,8,18]],"date-time":"2010-08-18T18:25:39Z","timestamp":1282155939000},"page":"817-829","source":"Crossref","is-referenced-by-count":20,"title":["ET<sub>A<\/sub> receptors are present in human aortic vascular endothelial cells and modulate intracellular calciumThis article is one of a selection of papers published in the two-part special issue entitled 20 Years of Endothelin Research."],"prefix":"10.1139","volume":"88","author":[{"given":"Levon","family":"Avedanian","sequence":"first","affiliation":[{"name":"Department of Anatomy and Cell Biology, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H5N4, Canada."},{"name":"Department of Pharmacology, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H5N4, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Riopel","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H5N4, Canada."},{"name":"Department of Pharmacology, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H5N4, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ghassan","family":"Bkaily","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H5N4, Canada."},{"name":"Department of Pharmacology, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H5N4, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moni","family":"Nader","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H5N4, Canada."},{"name":"Department of Pharmacology, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H5N4, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro","family":"D\u2019Orleans-Juste","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H5N4, Canada."},{"name":"Department of Pharmacology, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H5N4, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle","family":"Jacques","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H5N4, Canada."},{"name":"Department of Pharmacology, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H5N4, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"rg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1203\/01.PDR.0000160447.83332.13"},{"key":"rg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006840228104"},{"key":"rg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1139\/y03-020"},{"key":"rg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1139\/Y06-002"},{"key":"rg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1139\/Y08-048"},{"key":"rg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000142200"},{"key":"rg7\/ref7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.10.5573"},{"issue":"2","key":"rg8\/ref8","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/j.pharmthera.2005.11.001","volume":"111","author":"Brunner F.","year":"2006","journal-title":"Pharmacol. 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