{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T14:40:15Z","timestamp":1742827215897,"version":"3.40.2"},"reference-count":41,"publisher":"Canadian Science Publishing","issue":"4","license":[{"start":{"date-parts":[[2012,4,1]],"date-time":"2012-04-01T00:00:00Z","timestamp":1333238400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:p>Circulating autoantibodies directed against the 2nd extracellular loop (EL-2) of \u03b2<jats:sub>1<\/jats:sub>-adrenoceptors (\u03b2<jats:sub>1<\/jats:sub>-AABs) have been detected in the serum of patients with various cardiovascular pathologies. \u03b2<jats:sub>1<\/jats:sub>-AABs induce agonistic, positive inotropic effects via \u03b2<jats:sub>1<\/jats:sub>-adrenoceptors (\u03b2<jats:sub>1<\/jats:sub>ARs). In the mammalian heart, \u03b2<jats:sub>1<\/jats:sub>-AR can exist in 2 distinct activated configurations (the so-called high- and low-affinity states). The aim of the present study was to investigate whether the action of \u03b2<jats:sub>1<\/jats:sub>-AAB is dependent on the affinity state of \u03b2<jats:sub>1<\/jats:sub>AR in isolated ventricular cardiomyocytes of adult Wistar rats. Immunoglobulin G (IgG) containing \u03b2<jats:sub>1<\/jats:sub>-AAB obtained from animals immunized with a peptide corresponding to the EL-2 of human \u03b2<jats:sub>1<\/jats:sub>-AR, caused a dose-dependent increase in cell shortening. Isoproterenol-induced inotropy was significantly reduced in cardiomyocytes that had been preincubated with IgG containing \u03b2<jats:sub>1<\/jats:sub>-AAB and in cardiomyocytes isolated from immunized rats. The negative effects of preincubation with IgG containing \u03b2<jats:sub>1<\/jats:sub>-AAB on the response to isoproterenol was inhibited in the presence of bisoprolol. CGP 12177A and pindolol-induced inotropy was not affected by IgG preincubation or immunization. No detectable inotropic effect of cell shortening was obtained with IgG containing \u03b2<jats:sub>1<\/jats:sub>-AAB in the presence of propranolol and 3-isobutyl-1-methylxanthine. The present study demonstrates that \u03b2<jats:sub>1<\/jats:sub>-AABs have no agonist\/antagonist-like effects upon low-affinity state \u03b2<jats:sub>1<\/jats:sub>-ARs. This result indicates that \u03b2<jats:sub>1<\/jats:sub>-AABs recognize and stabilize the high-affinity state, but are unable to stabilize and (or) induce the low-affinity state receptor.<\/jats:p>","DOI":"10.1139\/y2012-006","type":"journal-article","created":{"date-parts":[[2012,3,30]],"date-time":"2012-03-30T13:22:19Z","timestamp":1333113739000},"page":"407-414","source":"Crossref","is-referenced-by-count":6,"title":["Autoantibodies against cardiac \u03b2<sub>1<\/sub>-adrenoceptor do not affect the low-affinity state \u03b2<sub>1<\/sub>-adrenoceptor-mediated inotropy in rat cardiomyocytes"],"prefix":"10.1139","volume":"90","author":[{"given":"Mohammed Amine","family":"Abdelkrim","sequence":"first","affiliation":[{"name":"L\u2019Universit\u00e9 Nantes Angers Le Mans (LUNAM) \u2013 Oniris, UPSP 5304 de physiopathologie animale et de pharmacologie fonctionnelle, Atlanpole-La Chantrerie, BP 40706, Nantes, F-44307, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed Yassine","family":"Mallem","sequence":"additional","affiliation":[{"name":"L\u2019Universit\u00e9 Nantes Angers Le Mans (LUNAM) \u2013 Oniris, UPSP 5304 de physiopathologie animale et de pharmacologie fonctionnelle, Atlanpole-La Chantrerie, BP 40706, Nantes, F-44307, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e9rard","family":"Chatagnon","sequence":"additional","affiliation":[{"name":"L\u2019Universit\u00e9 Nantes Angers Le Mans (LUNAM) \u2013 Oniris, Biotechnologie Pathologie Reproduction\/RSB, Atlanpole-La Chantrerie, BP 40706, Nantes, F-44307, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Gogny","sequence":"additional","affiliation":[{"name":"L\u2019Universit\u00e9 Nantes Angers Le Mans (LUNAM) \u2013 Oniris, UPSP 5304 de physiopathologie animale et de pharmacologie fonctionnelle, Atlanpole-La Chantrerie, BP 40706, Nantes, F-44307, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Claude","family":"Desfontis","sequence":"additional","affiliation":[{"name":"L\u2019Universit\u00e9 Nantes Angers Le Mans (LUNAM) \u2013 Oniris, UPSP 5304 de physiopathologie animale et de pharmacologie fonctionnelle, Atlanpole-La Chantrerie, BP 40706, Nantes, F-44307, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques","family":"Noireaud","sequence":"additional","affiliation":[{"name":"L\u2019Universit\u00e9 Nantes Angers Le Mans (LUNAM) \u2013 Oniris, UPSP 5304 de physiopathologie animale et de pharmacologie fonctionnelle, Atlanpole-La Chantrerie, BP 40706, Nantes, F-44307, France."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705991"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2008.045179"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(01)00225-5"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.2001.1414"},{"key":"refg5\/ref5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2005.pto_96607.x"},{"key":"refg6\/ref6","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1998.2510659.x"},{"issue":"2","key":"refg7\/ref7","doi-asserted-by":"crossref","first-page":"E199","DOI":"10.1152\/ajpendo.2001.280.2.E199","volume":"280","author":"Granneman J.G.","year":"2001","journal-title":"Am. 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