{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T19:49:20Z","timestamp":1751485760928,"version":"3.40.3"},"reference-count":40,"publisher":"Canadian Science Publishing","issue":"8","license":[{"start":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T00:00:00Z","timestamp":1343779200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:p>We have previously shown that A10 vascular smooth muscle cells (VSMC) exposed to angiotensin II (Ang\u2009II) exhibited overexpression of Gi\u03b1 proteins. In the present study, we examined the involvement of different signaling pathways in regulating Ang II induced enhanced expression of Gi\u03b1 proteins in VSMC by using pharmacological inhibitors. Ang II induced increased expression of Gi\u03b1 proteins in A10 VSMC was markedly attenuated by actinomycin D, losartan (an AT<jats:sub>1<\/jats:sub>receptor antagonist), dibutyryl cAMP, phospholipase C (PLC) inhibitor U73122, protein kinase C (PKC) inhibitors staurosporine and GP109203X, but not by PD123319 (an AT<jats:sub>2<\/jats:sub>receptor antagonist). In addition, BAPTA-AM and TMB-8 (chelators of intracellular Ca<jats:sup>2+<\/jats:sup>); and nifedipine (a blocker of L-type Ca<jats:sup>2+<\/jats:sup>channels) significantly inhibited Ang II induced enhanced expression of Gi\u03b1 proteins. On the other hand, extracellular Ca<jats:sup>2+<\/jats:sup>chelation using EGTA did not affect the Ang II evoked enhanced levels of Gi\u03b1 proteins. Furthermore, pretreatment of A10 VSMC with calmidazolium (an inhibitor of calmodulin), or KN93 (an inhibitor of CaM kinase), or genistein (an inhibitor of protein tyrosine kinase, PTK), also attenuated the increased levels of Gi\u03b1 proteins induced by Ang\u2009II. These results suggest that Ang II induced enhanced expression of Gi\u03b1 proteins may be regulated by different signaling pathways through AT<jats:sub>1<\/jats:sub>receptors in A10 VSMC.<\/jats:p>","DOI":"10.1139\/y2012-042","type":"journal-article","created":{"date-parts":[[2012,7,23]],"date-time":"2012-07-23T18:02:31Z","timestamp":1343066551000},"page":"1105-1116","source":"Crossref","is-referenced-by-count":8,"title":["Implication of multiple signaling pathways in the regulation of angiotensin II induced enhanced expression of Gi\u03b1 proteins in vascular smooth muscle cells"],"prefix":"10.1139","volume":"90","author":[{"given":"Yuan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, University of Montreal, C.P. 6128, Succursale centre-ville, Montreal, QC H3C\u00a03J7, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madhu B.","family":"Anand-Srivastava","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, University of Montreal, C.P. 6128, Succursale centre-ville, Montreal, QC H3C\u00a03J7, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.5.1617"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1139\/Y11-011"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(83)91217-2"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(89)90389-4"},{"issue":"1","key":"refg5\/ref5","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1042\/bj2880079","volume":"288","author":"Anand-Srivastava M.B.","year":"1992","journal-title":"Biochem. 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