{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T03:05:32Z","timestamp":1778295932619,"version":"3.51.4"},"reference-count":51,"publisher":"Canadian Science Publishing","issue":"9","license":[{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:p>d-Propranolol (d-Pro: 2\u20138\u00a0mg\u00b7(kg body mass)<jats:sup>\u20131<\/jats:sup>\u00b7day<jats:sup>\u20131<\/jats:sup>) protected against cardiac dysfunction and oxidative stress during 3\u20135\u00a0weeks of iron overload (2\u00a0mg Fe\u2013dextran\u00b7(g body mass)<jats:sup>\u20131<\/jats:sup>\u00b7week<jats:sup>\u20131<\/jats:sup>) in Sprague\u2013Dawley rats. At 3\u00a0weeks, hearts were perfused in working mode to obtain baseline function; red blood cell glutathione, plasma 8-isoprostane, neutrophil basal superoxide production, lysosomal-derived plasma N-acetyl-\u03b2-galactosaminidase (NAGA) activity, ventricular iron content, and cardiac iron deposition were assessed. Hearts from the Fe-treated group of rats exhibited lower cardiac work (26%) and output (CO, 24%); end-diastolic pressure rose 1.8-fold. Further, glutathione levels increased 2-fold, isoprostane levels increased 2.5-fold, neutrophil superoxide increased 3-fold, NAGA increased 4-fold, ventricular Fe increased 4.9-fold; and substantial atrial and ventricular Fe-deposition occurred. d-Pro (8\u00a0mg) restored heart function to the control levels, protected against oxidative stress, and decreased cardiac Fe levels. After 5\u00a0weeks of Fe treatment, echocardiography revealed that the following were depressed: percent fractional shortening (%FS, 31% lower); left ventricular (LV) ejection fraction (LVEF, 17%), CO (25%); and aortic pressure maximum (P<jats:sub>max<\/jats:sub>, 24%). Mitral valve E\/A declined by 18%, indicating diastolic dysfunction. Cardiac CD11b+ infiltrates were elevated. Low d-Pro (2\u00a0mg) provided modest protection, whereas 4\u20138\u00a0mg greatly improved LVEF (54%\u201375%), %FS (51%\u201381%), CO (43%\u201378%), P<jats:sub>max<\/jats:sub>(56%\u2013100%), and E\/A\u00a0&gt;100%; 8\u00a0mg decreased cardiac inflammation. Since d-Pro is an antioxidant and reduces cardiac Fe uptake as well as inflammation, these properties may preserve cardiac function during Fe overload.<\/jats:p>","DOI":"10.1139\/y2012-091","type":"journal-article","created":{"date-parts":[[2012,8,24]],"date-time":"2012-08-24T13:29:05Z","timestamp":1345814945000},"page":"1257-1268","source":"Crossref","is-referenced-by-count":25,"title":["<i>d<\/i>-Propranolol protects against oxidative stress and progressive cardiac dysfunction in iron overloaded rats"],"prefix":"10.1139","volume":"90","author":[{"given":"Jay H.","family":"Kramer","sequence":"first","affiliation":[{"name":"Biochemistry & Molecular Biology, Division of Experimental Medicine, 4th Floor Ross Hall, The George Washington University Medical Center, 2300 Eye Street, N.W, Washington, DC 20037, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher F.","family":"Spurney","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Children\u2019s National Heart Institute, Children\u2019s National Medical Center, Washington, DC 20010, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Micaela","family":"Iantorno","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Children\u2019s National Heart Institute, Children\u2019s National Medical Center, Washington, DC 20010, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Constantine","family":"Tziros","sequence":"additional","affiliation":[{"name":"Division of Cardiology, The George Washington University Medical Center, Washington, DC 20037, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna J.","family":"Chmielinska","sequence":"additional","affiliation":[{"name":"Biochemistry & Molecular Biology, Division of Experimental Medicine, 4th Floor Ross Hall, The George Washington University Medical Center, 2300 Eye Street, N.W, Washington, DC 20037, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. 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