{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T10:26:44Z","timestamp":1671618404944},"reference-count":41,"publisher":"Canadian Science Publishing","issue":"10","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p>Phosphatidylcholine (PC) is the major component of mammalian membranes, and the induction of PC biosynthesis has been shown to be an essential step in cell proliferation in various cell lines. Cytidine triphosphate (CTP):phosphocholine cytidylyltransferase \u03b1 (CT\u03b1) regulates the primary pathway of PC biosynthesis in the liver. The targeted disruption of CT\u03b1 in murine liver (LCT\u03b1<jats:sup>\u2212\/\u2212<\/jats:sup>mice) decreases hepatic PC mass and the number of cells in the liver, suggesting CT\u03b1 as an important factor for hepatocyte proliferation. To elucidate the role of CT\u03b1 in hepatic cell division in vivo, we monitored liver regeneration after 70% partial hepatectomy in LCT\u03b1<jats:sup>\u2212\/\u2212<\/jats:sup>and loxP flanked (floxed) LCT\u03b1 (control) mice. To our surprise, liver re-growth, DNA synthesis, and PC mass after surgery were not impaired in LCT\u03b1<jats:sup>\u2212\/\u2212<\/jats:sup>mice, despite reduced total PC synthesis. Furthermore, PC synthesis in the control mice was not induced after 70% partial hepatectomy. We conclude that CT\u03b1 is not essential for proliferation of hepatocytes in vivo, and that basal hepatic PC biosynthesis is sufficient to sustain regeneration after 70% partial hepatectomy.<\/jats:p>","DOI":"10.1139\/y2012-116","type":"journal-article","created":{"date-parts":[[2012,10,22]],"date-time":"2012-10-22T18:09:19Z","timestamp":1350929359000},"page":"1403-1412","source":"Crossref","is-referenced-by-count":3,"title":["Impaired phosphatidylcholine biosynthesis does not attenuate liver regeneration after 70% partial hepatectomy in hepatic CTP:phosphocholine cytidylyltransferase-\u03b1 deficient mice"],"prefix":"10.1139","volume":"90","author":[{"given":"Ji","family":"Ling","sequence":"first","affiliation":[{"name":"Group on the Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, AB T6G\u00a02S2, Canada."},{"name":"Department of Biochemistry, University of Alberta, Edmonton, AB T6G\u00a02S2, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin Fu","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Alberta, Edmonton, AB T6G\u00a02S2, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis E.","family":"Vance","sequence":"additional","affiliation":[{"name":"Group on the Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, AB T6G\u00a02S2, Canada."},{"name":"Department of Biochemistry, University of Alberta, Edmonton, AB T6G\u00a02S2, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren\u00e9 L.","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Group on the Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, AB T6G\u00a02S2, Canada."},{"name":"Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, AB T6G\u00a02S2, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","reference":[{"key":"refg1\/ref1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304810200"},{"key":"refg2\/ref2","doi-asserted-by":"publisher","DOI":"10.1038\/laban1108-529"},{"key":"refg3\/ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(77)90009-X"},{"key":"refg4\/ref4","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.25.14668"},{"issue":"1","key":"refg5\/ref5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0021-9258(19)40184-1","volume":"265","author":"Exton J.H.","year":"1990","journal-title":"J. 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