{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,4]],"date-time":"2023-11-04T15:26:40Z","timestamp":1699111600628},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"10","license":[{"start":{"date-parts":[[1979,10,1]],"date-time":"1979-10-01T00:00:00Z","timestamp":307584000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1979,10,1]]},"abstract":"<jats:p> Myelin isolated from the rat peripheral nervous system (sciatic nerve and cauda equina) contained Mg<jats:sup>2+<\/jats:sup>-dependent protein kinase that phosphorylated myelin polypeptides. Ca<jats:sup>2+<\/jats:sup>, in micromolar concentrations, markedly stimulated phosphorylation (half-maximal stimulation at 5\u2002\u03bcM (free) Ca<jats:sup>2+<\/jats:sup>) but at higher concentrations (&gt;\u2002100\u2002\u03bcM Ca<jats:sup>2+<\/jats:sup>) it caused inhibition. In the presence of Triton X-100, phosphorylation (\u00b1Ca<jats:sup>2+<\/jats:sup>) of myelin was increased and Ca<jats:sup>2+<\/jats:sup> caused up to a 10-fold increase in phosphorylation. Among the myelin polypeptides, P<jats:sub>0<\/jats:sub> (M<jats:sub>r<\/jats:sub>, 28\u2002000), a major glycoprotein, accounted for nearly 60% of the total phosphate incorporated into the myelin and Ca<jats:sup>2+<\/jats:sup> markedly promoted phosphorylation of P<jats:sub>0<\/jats:sub>. Phosphorylation of other myelin polypeptides, P<jats:sub>2<\/jats:sub> (M<jats:sub>r<\/jats:sub>, 16\u2002000), Y (M<jats:sub>r<\/jats:sub>, 26\u2002000), and P<jats:sub>1<\/jats:sub> (M<jats:sub>r<\/jats:sub>, 20\u2002000), and the Ca<jats:sup>2+<\/jats:sup>-stimulatory effect on phosphorylation of these were also evident. Cyclic AMP (or other cyclic nucleotides) failed to show any significant stimulatory effect on myelin phosphorylation. <\/jats:p>","DOI":"10.1139\/y79-181","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T04:51:49Z","timestamp":1303620709000},"page":"1200-1204","source":"Crossref","is-referenced-by-count":17,"title":["Calcium ion stimulated endogenous protein kinase catalyzed phosphorylation of peripheral nerve myelin proteins"],"prefix":"10.1139","volume":"57","author":[{"given":"Elena H.","family":"Petrali","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prakash V.","family":"Sulakhe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y79-181","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T11:18:44Z","timestamp":1578223124000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y79-181"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,10,1]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1979,10,1]]}},"alternative-id":["10.1139\/y79-181"],"URL":"https:\/\/doi.org\/10.1139\/y79-181","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,10,1]]}}}