{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T21:42:02Z","timestamp":1757540522020},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"2","license":[{"start":{"date-parts":[[1980,2,1]],"date-time":"1980-02-01T00:00:00Z","timestamp":318211200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1980,2,1]]},"abstract":"<jats:p> Astrocytes in primary cultures were found to bind large amounts of [<jats:sup>3<\/jats:sup>H]diazepam. More than 90% of the total binding is specific, i.e., displaceable by an excess of nonlabeled diazepam. At saturation the specific binding amounts to about 20\u2002pmol\/mg protein and the K<jats:sub>d<\/jats:sub> value is 30\u2002nM. Clonazepam and flurazepam displace [<jats:sup>3<\/jats:sup>H]diazepam from its specific binding sites; 20\u201330% displacement is brought about by 0.1\u20130.3\u2002\u03bcM clonazepam, which is similar to the plasma concentrations encountered in epileptic patients treated with this drug. Barbiturates have a similar effect, but considerably higher concentrations are required. <\/jats:p>","DOI":"10.1139\/y80-036","type":"journal-article","created":{"date-parts":[[2011,4,23]],"date-time":"2011-04-23T16:47:04Z","timestamp":1303577224000},"page":"217-220","source":"Crossref","is-referenced-by-count":23,"title":["Diazepam receptors on mouse astrocytes in primary cultures: displacement by pharmacologically active concentrations of benzodiazepines or barbiturates"],"prefix":"10.1139","volume":"58","author":[{"given":"Leif","family":"Hertz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srimathie","family":"Mukerji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y80-036","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T11:18:51Z","timestamp":1578223131000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y80-036"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1980,2,1]]}},"alternative-id":["10.1139\/y80-036"],"URL":"https:\/\/doi.org\/10.1139\/y80-036","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,2,1]]}}}