{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T13:00:39Z","timestamp":1649163639582},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"8","license":[{"start":{"date-parts":[[1982,8,1]],"date-time":"1982-08-01T00:00:00Z","timestamp":397008000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1982,8,1]]},"abstract":"<jats:p> The cellular sources and gonadotropic regulation of porcine ovarian estrogen and androgen were assessed by culturing isolated granulosa cells and thecal cells from medium size follicles (4\u20136\u2002mm diameter) separately for 24\u2002h in a chemically defined medium containing gonadotropins and (or) testosterone. At the end of the culture period, estradiol-17\u03b2 (estradiol) and androgens in the media were determined by radioimmunoassays. Production of estradiol by granulosa cells without an exogenous aromatizable androgen was low in the absence or presence of a highly purified preparation of either follicle-stimulating hormone (FSH, 0.25\u2002\u03bcg\/mL) or luteinizing hormone (LH, 1\u2002\u03bcg\/mL). Addition of testosterone or androstenedione (0.5\u2002\u03bcM), but not dihydrotestosterone or pregnenolone, significantly increased estradiol secretion. Additional increases were observed when FSH, LH, prostaglandin E<jats:sub>2<\/jats:sub>, or dibutyryl cyclic 3\u2032,5\u2032-adenosine monophosphate was present. Production of estradiol by thecal cells was low in the presence or absence of exogenous testosterone, and was essentially unaffected by the presence of gonadotropins. Thecal cells, however, released large amounts of androstenedione and smaller amounts of testosterone and other androgens during 24-h culture and the production of these androgens was stimulated by LH but not by FSH. Androgen secretion by granulosa cells was negligible when compared with the theca and was unaffected by gonadotropins. It is concluded that the theca is the prime site for follicular androgen biosynthesis by the porcine ovarian follicle, and, upon LH stimulation, may provide androgen precursors for estradiol production by granulosa cells. <\/jats:p>","DOI":"10.1139\/y82-160","type":"journal-article","created":{"date-parts":[[2011,4,23]],"date-time":"2011-04-23T20:48:51Z","timestamp":1303591731000},"page":"1112-1118","source":"Crossref","is-referenced-by-count":3,"title":["Estradiol-17\u03b2 and androgen secretion by isolated porcine ovarian follicular cells <i>in vitro<\/i>"],"prefix":"10.1139","volume":"60","author":[{"given":"B. K.","family":"Tsang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. S.","family":"Moon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. T.","family":"Armstrong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y82-160","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T16:22:26Z","timestamp":1578241346000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y82-160"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,8,1]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1982,8,1]]}},"alternative-id":["10.1139\/y82-160"],"URL":"https:\/\/doi.org\/10.1139\/y82-160","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,8,1]]}}}