{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:06:30Z","timestamp":1761059190875},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"8","license":[{"start":{"date-parts":[[1983,8,1]],"date-time":"1983-08-01T00:00:00Z","timestamp":428544000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1983,8,1]]},"abstract":"<jats:p> A method was developed to study sickling in vitro under physiological conditions using a small amount of blood (0.1\u2002mL). The diluted blood suspension (2.1\u2002mL) was placed in a flask and flushed with a gas mixture containing 5% CO<jats:sub>2<\/jats:sub>. In deoxygenation experiments, samples were withdrawn anaerobically into a microslide (optical path 0.1\u2002mm) and red cell morphology was studied directly under a light microscope after both ends of the microslide were sealed. The blood suspension with a hematocrit value of 1% can be deoxygenated in less than 10\u2002min, but it takes 30\u2002min for the sickling of cells to reach a plateau. The degree of sickling increases with increasing osmolality of the medium, or with a decrease of the pH. With a citrate\u2013phosphate\u2013dextrose\u2013adenine solution, sickle blood may be stored for this type of study for about 10 days at 4 \u00b0C. The blood may be stored for about 5 days with a noncitrate preservative. This method was found useful in examining the antisickling activity of various drugs. <\/jats:p>","DOI":"10.1139\/y83-140","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T08:52:39Z","timestamp":1303635159000},"page":"941-945","source":"Crossref","is-referenced-by-count":10,"title":["Measurement of red cell sickling: a method for studying the efficacy of antisickling drugs under physiological conditions"],"prefix":"10.1139","volume":"61","author":[{"given":"S. Tsuyoshi","family":"Ohnishi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Hashimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y83-140","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T16:22:19Z","timestamp":1578241339000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y83-140"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,8,1]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1983,8,1]]}},"alternative-id":["10.1139\/y83-140"],"URL":"https:\/\/doi.org\/10.1139\/y83-140","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,8,1]]}}}