{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T13:54:25Z","timestamp":1723038865138},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"11","license":[{"start":{"date-parts":[[1983,11,1]],"date-time":"1983-11-01T00:00:00Z","timestamp":436492800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1983,11,1]]},"abstract":"<jats:p> Synthetic bovine parathyroid hormone fragment containing the N-terminal 1 \u2013 34 amino acids (bPTH-(1 \u2013 34)) relaxed the guinea-pig trachea constricted with histamine in vitro. Peptides with bovine and human sequences purchased from Peninsula Laboratories and Beckman Bioproducts produced similar effects. Substitution of methionine in positions 8 and 18 by norleucine did not affect this property of bPTH-(1 \u2013 34). However, when the methionines were oxidized by treating the peptide with hydrogen peroxide, the peptide could no longer produce relaxation in the trachea. Oxidation of the methionine-replaced analog did not affect the action of the peptide on the trachea. It seems that the methionines per se are not necessary, but once oxidized the conformation of the molecule may be sufficiently altered to affect its ability to relax the trachea. While propranolol can block the relaxing action of isoproterenol, this blocking agent produces no inhibition of the bPTH-(1 \u2013 34) effect. This action of PTH on the trachea may be related to cAMP because isobutyryl-methylxanthine, a phosphodiesterase inhibitor, potentiates and imidazole, a phosphodiesterase stimulator, inhibits the trachea relaxing action of bPTH-(1 \u2013 34). <\/jats:p>","DOI":"10.1139\/y83-191","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T04:52:50Z","timestamp":1303620770000},"page":"1324-1328","source":"Crossref","is-referenced-by-count":13,"title":["Parathyroid hormone (PTH) fragments relax the guinea-pig trachea <i>in vitro<\/i>"],"prefix":"10.1139","volume":"61","author":[{"given":"Y. C.","family":"Yen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"May C. M.","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander D.","family":"Kenny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter K. T.","family":"Pang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y83-191","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T11:22:35Z","timestamp":1578223355000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y83-191"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,11,1]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1983,11,1]]}},"alternative-id":["10.1139\/y83-191"],"URL":"https:\/\/doi.org\/10.1139\/y83-191","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,11,1]]}}}