{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T11:27:01Z","timestamp":1758281221663},"reference-count":0,"publisher":"Canadian Science Publishing","issue":"5","license":[{"start":{"date-parts":[[1985,5,1]],"date-time":"1985-05-01T00:00:00Z","timestamp":483753600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.nrcresearchpress.com\/page\/about\/CorporateTextAndDataMining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Can. J. Physiol. Pharmacol."],"published-print":{"date-parts":[[1985,5,1]]},"abstract":"<jats:p> Methyltetrazolethiol (1-methyl-5-mercapto-1,2,3,4-tetrazole, MTT) is a heterocyclic substituent of the cephalosporin antibiotics, cefamandole, cefoperazone, and moxalactam. Pretreatment of rats with MTT has been reported to increase blood acetaldehyde concentration after ethanol administration. The time course of MTT-induced inhibition of hepatic aldehyde dehydrogenases (ALDH) was determined in adult, male Sprague\u2013Dawley rats in comparison with the hepatic ALDH inhibition induced by calcium carbimide (calcium cyanamide, CC) and disulfiram (D). The apparent onset of maximal inhibition of hepatic low K<jats:sub>m<\/jats:sub> ALDH occurred at 2\u2002h for 50\u2002mg\/kg MTT (subcutaneous, s.c.) and 7\u2002mg\/kg CC (oral) and at 24\u2002h for 300\u2002mg\/kg D (oral). The relative magnitude of maximal inhibition of low K<jats:sub>m<\/jats:sub> ALDH was CC\u2002&gt;\u2002D\u2002&gt;\u2002MTT. The relative duration of enzyme inhibition was D\u2002&gt;\u2002MTT\u2002&gt;\u2002CC. High K<jats:sub>m<\/jats:sub> ALDH was only inhibited by CC. Hepatic low K<jats:sub>m<\/jats:sub> ALDH was selectively inhibited by s.c. and oral administration of 125\u2002mg\/kg MTT. For s.c. administration of 125\u2002mg\/kg MTT, the magnitude of maximal enzyme inhibition and the duration of inhibition were greater than for the 50\u2002mg\/kg dose. Oral administration of 125\u2002mg\/kg MTT produced similar inhibition of hepatic low K<jats:sub>m<\/jats:sub> ALDH compared with s.c. administration of the same dose. The time course of blood ethanol and acetaldehyde concentrations was determined for the intravenous infusion of two 0.3-g\/kg doses of ethanol to rats that were pretreated orally with saline (1\u2002h), MTT (125\u2002mg\/kg, 2\u2002h), or CC (7\u2002mg\/kg, 1\u2002h). The relative increase in blood acetaldehyde concentration compared with saline pretreatment was CC\u2002&gt;\u2002MTT. The elimination of ethanol from blood was slower in the MTT- and CC-pretreated animals, and this effect was more pronounced for CC pretreatment. Overall, the data demonstrate that the characteristics of hepatic ALDH inhibition for MTT are different from those of the known ALDH inhibitors, CC and D. <\/jats:p>","DOI":"10.1139\/y85-076","type":"journal-article","created":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T08:53:25Z","timestamp":1303635205000},"page":"438-443","source":"Crossref","is-referenced-by-count":15,"title":["A comparative study of the inhibition of hepatic aldehyde dehydrogenases in the rat by methyltetrazolethiol, calcium carbimide, and disulfiram"],"prefix":"10.1139","volume":"63","author":[{"given":"J. F.","family":"Brien","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. S.","family":"Tam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J.","family":"Cameron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. A. E.","family":"Steenaart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. W.","family":"Loomis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"155","container-title":["Canadian Journal of Physiology and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/pdf\/10.1139\/y85-076","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T16:21:53Z","timestamp":1578241313000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.nrcresearchpress.com\/doi\/10.1139\/y85-076"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1985,5,1]]}},"alternative-id":["10.1139\/y85-076"],"URL":"https:\/\/doi.org\/10.1139\/y85-076","relation":{},"ISSN":["0008-4212","1205-7541"],"issn-type":[{"value":"0008-4212","type":"print"},{"value":"1205-7541","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,5,1]]}}}